Perspectives and benefits of high-throughput long-read sequencing in microbial ecology

L Tedersoo, M Albertsen, S Anslan… - Applied and …, 2021 - Am Soc Microbiol
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

[HTML][HTML] Monitoring antibiotic resistance genes in wastewater environments: the challenges of filling a gap in the One-Health cycle

A Miłobedzka, C Ferreira, I Vaz-Moreira… - Journal of Hazardous …, 2022 - Elsevier
Antibiotic resistance (AR) is a global problem requiring international cooperation and
coordinated action. Global monitoring must rely on methods available and comparable …

BiG-SLiCE: A highly scalable tool maps the diversity of 1.2 million biosynthetic gene clusters

SA Kautsar, JJJ van der Hooft, D de Ridder… - …, 2021 - academic.oup.com
Background Genome mining for biosynthetic gene clusters (BGCs) has become an integral
part of natural product discovery. The> 200,000 microbial genomes now publicly available …

Candidatus Dechloromonas phosphoritropha” and “Ca. D. phosphorivorans”, novel polyphosphate accumulating organisms abundant in wastewater treatment …

F Petriglieri, C Singleton, M Peces… - The ISME …, 2021 - academic.oup.com
Members of the genus Dechloromonas are often abundant in enhanced biological
phosphorus removal (EBPR) systems and are recognized putative polyphosphate …

Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors

P Bovio-Winkler, LD Guerrero, L Erijman, P Oyarzúa… - BMC microbiology, 2023 - Springer
Abstract Background The phylum Chloroflexi is highly abundant in a wide variety of
wastewater treatment bioreactors. It has been suggested that they play relevant roles in …

Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing

V Waschulin, C Borsetto, R James… - The ISME …, 2022 - academic.oup.com
The growing problem of antibiotic resistance has led to the exploration of uncultured
bacteria as potential sources of new antimicrobials. PCR amplicon analyses and short-read …

Recent advances in understanding the ecophysiology of enhanced biological phosphorus removal

S Roy, Q Guanglei, R Zuniga-Montanez… - Current Opinion in …, 2021 - Elsevier
Highlights•Recently identified putative PAOs reveal vast metabolic versatility compared to
Accumulibacter.•Electron donors and acceptors drive EBPR community composition and …

[HTML][HTML] Comparing the significance of the utilization of next generation and third generation sequencing technologies in microbial metagenomics

I Akacin, Ş Ersoy, O Doluca, M Güngörmüşler - Microbiological Research, 2022 - Elsevier
Since the exploration of sequencing began in 2005, third and next-generation sequencing
(TGS and NGS) technologies have fundamentally changed metagenomics research. These …

Global warming readiness: feasibility of enhanced biological phosphorus removal at 35° C

G Qiu, Y Law, R Zuniga-Montanez, X Deng, Y Lu… - Water Research, 2022 - Elsevier
Recent research has shown enhanced biological phosphorus removal (EBPR) from
municipal wastewater at warmer temperatures around 30° C to be achievable in both …

uBin: A manual refining tool for genomes from metagenomes

TLV Bornemann, SP Esser, TL Stach… - Environmental …, 2023 - Wiley Online Library
Resolving bacterial and archaeal genomes from metagenomes has revolutionized our
understanding of Earth's biomes yet producing high‐quality genomes from assembled …