The triumphs and limitations of computational methods for scRNA-seq

PV Kharchenko - Nature methods, 2021 - nature.com
The rapid progress of protocols for sequencing single-cell transcriptomes over the past
decade has been accompanied by equally impressive advances in the computational …

Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment

G Huang, F Li, X Zhao, Y Ma, Y Li, M Lin, G **… - Chemical …, 2017 - ACS Publications
The cell microenvironment has emerged as a key determinant of cell behavior and function
in development, physiology, and pathophysiology. The extracellular matrix (ECM) within the …

Increased glucose metabolism in TAMs fuels O-GlcNAcylation of lysosomal Cathepsin B to promote cancer metastasis and chemoresistance

Q Shi, Q Shen, Y Liu, Y Shi, W Huang, X Wang, Z Li… - Cancer cell, 2022 - cell.com
How glucose metabolism remodels pro-tumor functions of tumor-associated macrophages
(TAMs) needs further investigation. Here we show that M2-like TAMs bear the highest …

T cell characteristics associated with toxicity to immune checkpoint blockade in patients with melanoma

AX Lozano, AA Chaudhuri, A Nene, A Bacchiocchi… - Nature medicine, 2022 - nature.com
Severe immune-related adverse events (irAEs) occur in up to 60% of patients with
melanoma treated with immune checkpoint inhibitors (ICIs). However, it is unknown whether …

[HTML][HTML] Complete map** of mutations to the SARS-CoV-2 spike receptor-binding domain that escape antibody recognition

AJ Greaney, TN Starr, P Gilchuk, SJ Zost, E Binshtein… - Cell host & …, 2021 - cell.com
Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being
developed as therapeutics and are a major contributor to neutralizing antibody responses …

Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy

R Yang, L Sun, CF Li, YH Wang, J Yao, H Li… - Nature …, 2021 - nature.com
The two T cell inhibitory receptors PD-1 and TIM-3 are co-expressed during exhausted T cell
differentiation, and recent evidence suggests that their crosstalk regulates T cell exhaustion …

CellRank for directed single-cell fate map**

M Lange, V Bergen, M Klein, M Setty, B Reuter… - Nature …, 2022 - nature.com
Computational trajectory inference enables the reconstruction of cell state dynamics from
single-cell RNA sequencing experiments. However, trajectory inference requires that the …

Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients

J Hadjadj, N Yatim, L Barnabei, A Corneau, J Boussier… - Science, 2020 - science.org
Coronavirus disease 2019 (COVID-19) is characterized by distinct patterns of disease
progression that suggest diverse host immune responses. We performed an integrated …

[HTML][HTML] Comprehensive profiling of an aging immune system reveals clonal GZMK+ CD8+ T cells as conserved hallmark of inflammaging

DA Mogilenko, O Shpynov, PS Andhey, L Arthur… - Immunity, 2021 - cell.com
Systematic understanding of immune aging on a whole-body scale is currently lacking. We
characterized age-associated alterations in immune cells across multiple mouse organs …

Understanding how dimension reduction tools work: an empirical approach to deciphering t-SNE, UMAP, TriMAP, and PaCMAP for data visualization

Y Wang, H Huang, C Rudin, Y Shaposhnik - Journal of Machine Learning …, 2021 - jmlr.org
Dimension reduction (DR) techniques such as t-SNE, UMAP, and TriMap have
demonstrated impressive visualization performance on many real-world datasets. One …