Imaging technologies for flow cytometry

Y Han, Y Gu, AC Zhang, YH Lo - Lab on a Chip, 2016 - pubs.rsc.org
High-throughput single cell imaging is a critical enabling and driving technology in
molecular and cellular biology, biotechnology, medicine and related areas. Imaging flow …

AI on a chip

A Isozaki, J Harmon, Y Zhou, S Li, Y Nakagawa… - Lab on a Chip, 2020 - pubs.rsc.org
Artificial intelligence (AI) has dramatically changed the landscape of science, industry,
defence, and medicine in the last several years. Supported by considerably enhanced …

Real-time fluorescence and deformability cytometry

P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner… - Nature …, 2018 - nature.com
The throughput of cell mechanical characterization has recently approached that of
conventional flow cytometers. However, this very sensitive, label-free approach still lacks the …

High-throughput imaging flow cytometry by optofluidic time-stretch microscopy

C Lei, H Kobayashi, Y Wu, M Li, A Isozaki… - Nature protocols, 2018 - nature.com
The ability to rapidly assay morphological and intracellular molecular variations within large
heterogeneous populations of cells is essential for understanding and exploiting cellular …

Review and perspectives on microfluidic flow cytometers

RJ Yang, LM Fu, HH Hou - Sensors and Actuators B: Chemical, 2018 - Elsevier
Modern microflow cytometers are sophisticated instruments capable of measuring multiple
physical characteristics of a single cell simultaneously as the cells flow in suspension …

Intelligent classification of platelet aggregates by agonist type

Y Zhou, A Yasumoto, C Lei, CJ Huang, H Kobayashi… - Elife, 2020 - elifesciences.org
Platelets are anucleate cells in blood whose principal function is to stop bleeding by forming
aggregates for hemostatic reactions. In addition to their participation in physiological …

Perspective on quantitative phase imaging to improve precision cancer medicine

Y Liu, S Uttam - Journal of biomedical optics, 2024 - spiedigitallibrary.org
Significance Quantitative phase imaging (QPI) offers a label-free approach to non-invasively
characterize cellular processes by exploiting their refractive index based intrinsic contrast …

A practical guide to intelligent image-activated cell sorting

A Isozaki, H Mikami, K Hiramatsu, S Sakuma, Y Kasai… - Nature protocols, 2019 - nature.com
Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that
performs real-time intelligent image-based sorting of single cells with high throughput. iIACS …

Elevating chemistry research with a modern electronics toolkit

GRD Prabhu, PL Urban - Chemical Reviews, 2020 - ACS Publications
With the rapid development of high technology, chemical science is not as it used to be a
century ago. Many chemists acquire and utilize skills that are well beyond the traditional …

Label-free detection of cellular drug responses by high-throughput bright-field imaging and machine learning

H Kobayashi, C Lei, Y Wu, A Mao, Y Jiang, B Guo… - Scientific reports, 2017 - nature.com
In the last decade, high-content screening based on multivariate single-cell imaging has
been proven effective in drug discovery to evaluate drug-induced phenotypic variations …