Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Mitochondria are central hubs of cellular metabolism and are tightly connected to signaling
pathways. The dynamic plasticity of mitochondria to fuse, divide, and contact other …

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by
a lipid bilayer from which spike (S) protein trimers protrude. Heavily glycosylated S trimers …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Activating mutations in leucine-rich repeat kinase 2 (LRRK2) represent the most common
cause of monogenic Parkinson's disease. LRRK2 is a large multidomain protein kinase that …

Structural biology studies inside cells and tissues require methods to thin vitrified specimens
to electron transparency. Until now, focused ion beams based on gallium have been used …

The eukaryotic endomembrane system consists of multiple interconnected organelles. Rab
GTPases are organelle-specific markers that give identity to these membranes by recruiting …

The Commander complex is required for endosomal recycling of diverse transmembrane
cargos and is mutated in Ritscher-Schinzel syndrome. It comprises two sub-assemblies …

Bacteria encode myriad defences that target the genomes of infecting bacteriophage,
including restriction–modification and CRISPR–Cas systems. In response, one family of …

Endosomes are dynamic intracellular compartments that control the sorting of a constant
stream of different transmembrane cargos either for ESCRT‐mediated degradation or for …