Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global public
health crisis. Effective COVID-19 vaccines developed by Pfizer-BioNTech, Moderna, and …
health crisis. Effective COVID-19 vaccines developed by Pfizer-BioNTech, Moderna, and …
Potent neutralization of SARS-CoV-2 variants by RBD nanoparticle and prefusion-stabilized spike immunogens
We previously described a two-component protein nanoparticle vaccine platform that
displays 60 copies of the SARS-CoV-2 spike protein RBD (RBD-NP). The vaccine, when …
displays 60 copies of the SARS-CoV-2 spike protein RBD (RBD-NP). The vaccine, when …
Neutralizing efficacy of encapsulin nanoparticles against SARS-CoV2 variants of concern
Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of
existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine …
existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine …
Mutations in S2 subunit of SARS-CoV-2 Omicron spike strongly influence its conformation, fusogenicity, and neutralization sensitivity
ABSTRACT SARS-CoV-2 has a remarkable ability to respond to and evolve against the
selection pressure by host immunity exemplified by the emergence of Omicron lineage …
selection pressure by host immunity exemplified by the emergence of Omicron lineage …
Mechanistic insights into global suppressors of protein folding defects
Most amino acid substitutions in a protein either lead to partial loss-of-function or are near
neutral. Several studies have shown the existence of second-site mutations that can rescue …
neutral. Several studies have shown the existence of second-site mutations that can rescue …
Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries
Although there have been recent transformative advances in the area of protein structure
prediction, prediction of point mutations that improve protein stability remains challenging. It …
prediction, prediction of point mutations that improve protein stability remains challenging. It …
Mammalian cell expressed recombinant trimeric spike protein is a potent vaccine antigen and confers near-complete protection against SARS-CoV-2 infection in …
Numerous vaccine candidates have emerged in the fight against SARS-CoV-2, yet the
challenges posed by viral evolution and the evasion of vaccine-induced immunity persist …
challenges posed by viral evolution and the evasion of vaccine-induced immunity persist …
Scalable bioprocess for high-yield production of SARS-CoV-2 trimeric spike protein-based immunogen (IMT-CVAX) using suspension CHO cells
The COVID-19 pandemic has brought global attention towards the readiness of
biotechnology platforms for rapid development of immunogens to be used as vaccine …
biotechnology platforms for rapid development of immunogens to be used as vaccine …
Highly thermotolerant sars-cov-2 vaccine elicits neutralising antibodies against delta and omicron in mice
As existing vaccines fail to completely prevent COVID-19 infections or community
transmission, there is an unmet need for vaccines that can better combat SARS-CoV-2 …
transmission, there is an unmet need for vaccines that can better combat SARS-CoV-2 …
Improved prediction of stabilizing mutations in proteins by incorporation of mutational effects on ligand binding
S Ganesan, N Mittal, A Bhat, RS Adiga… - Proteins: Structure …, 2025 - Wiley Online Library
While many computational methods accurately predict destabilizing mutations, identifying
stabilizing mutations has remained a challenge, because of their relative rarity. We tested …
stabilizing mutations has remained a challenge, because of their relative rarity. We tested …