Controlling the sound of light: photoswitching optoacoustic imaging

AC Stiel, V Ntziachristos - Nature Methods, 2024 - nature.com
Optoacoustic (photoacoustic) imaging advances allow high-resolution optical imaging much
deeper than optical microscopy. However, while label-free optoacoustics have already …

Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools

DM Shcherbakova, AA Shemetov… - Annual review of …, 2015 - annualreviews.org
Genetically encoded optical tools have revolutionized modern biology by allowing detection
and control of biological processes with exceptional spatiotemporal precision and sensitivity …

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

DM Shcherbakova, M Baloban, AV Emelyanov… - Nature …, 2016 - nature.com
Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein
tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We …

Signal amplification and transduction in phytochrome photosensors

H Takala, A Björling, O Berntsson, H Lehtivuori… - Nature, 2014 - nature.com
Sensory proteins must relay structural signals from the sensory site over large distances to
regulatory output domains. Phytochromes are a major family of red-light-sensing kinases …

The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser

E Claesson, WY Wahlgren, H Takala, S Pandey… - Elife, 2020 - elifesciences.org
Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi,
and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to …

Protein control of photochemistry and transient intermediates in phytochromes

G Salvadori, V Macaluso, G Pellicci, L Cupellini… - Nature …, 2022 - nature.com
Phytochromes are ubiquitous photoreceptors responsible for sensing light in plants, fungi
and bacteria. Their photoactivation is initiated by the photoisomerization of the embedded …

Near-infrared fluorescent proteins engineered from bacterial phytochromes

DM Shcherbakova, M Baloban… - Current opinion in chemical …, 2015 - Elsevier
Highlights•Available phenotypes and photochemical properties of near-infrared fluorescent
proteins.•Applications of near-infrared fluorescent proteins in basic and translational …

Structural photoactivation of a full-length bacterial phytochrome

A Björling, O Berntsson, H Lehtivuori, H Takala… - Science …, 2016 - science.org
Phytochromes are light sensor proteins found in plants, bacteria, and fungi. They function by
converting a photon absorption event into a conformational signal that propagates from the …

How to increase brightness of near-infrared fluorescent proteins in mammalian cells

AA Shemetov, OS Oliinyk, VV Verkhusha - Cell chemical biology, 2017 - cell.com
Numerous near-infrared (NIR) fluorescent proteins (FPs) were recently engineered from
bacterial photoreceptors but lack of their systematic comparison makes researcher's choice …

On the (un) coupling of the chromophore, tongue interactions, and overall conformation in a bacterial phytochrome

H Takala, HK Lehtivuori, O Berntsson, A Hughes… - Journal of Biological …, 2018 - ASBMB
Phytochromes are photoreceptors in plants, fungi, and various microorganisms and cycle
between metastable red light–absorbing (Pr) and far-red light–absorbing (Pfr) states. Their …