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Machine learning for functional protein design
Recent breakthroughs in AI coupled with the rapid accumulation of protein sequence and
structure data have radically transformed computational protein design. New methods …
structure data have radically transformed computational protein design. New methods …
Droplet-based microfluidics
T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
Real-time detection of 20 amino acids and discrimination of pathologically relevant peptides with functionalized nanopore
Precise identification and quantification of amino acids is crucial for many biological
applications. Here we report a copper (II)-functionalized Mycobacterium smegmatis porin A …
applications. Here we report a copper (II)-functionalized Mycobacterium smegmatis porin A …
An introduction to mass spectrometry-based proteomics
SR Shuken - Journal of proteome research, 2023 - ACS Publications
Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …
proteome. Because the foundations of mass spectrometry-based proteomics are complex …
Peptide sequencing based on host–guest interaction-assisted nanopore sensing
Direct protein sequencing technologies with improved sensitivity and throughput are still
needed. Here, we propose an alternative method for peptide sequencing based on …
needed. Here, we propose an alternative method for peptide sequencing based on …
Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …
Multiple rereads of single proteins at single–amino acid resolution using nanopores
H Brinkerhoff, ASW Kang, J Liu, A Aksimentiev… - Science, 2021 - science.org
A proteomics tool capable of identifying single proteins would be important for cell biology
research and applications. Here, we demonstrate a nanopore-based single-molecule …
research and applications. Here, we demonstrate a nanopore-based single-molecule …
Spatially resolved single-cell translatomics at molecular resolution
The precise control of messenger RNA (mRNA) translation is a crucial step in
posttranscriptional gene regulation of cellular physiology. However, it remains a challenge …
posttranscriptional gene regulation of cellular physiology. However, it remains a challenge …
Multi-pass, single-molecule nanopore reading of long protein strands
K Motone, D Kontogiorgos-Heintz, J Wee, K Kurihara… - Nature, 2024 - nature.com
The ability to sequence single protein molecules in their native, full-length form would
enable a more comprehensive understanding of proteomic diversity. Current technologies …
enable a more comprehensive understanding of proteomic diversity. Current technologies …
Proteomic landscape of Alzheimer's Disease: novel insights into pathogenesis and biomarker discovery
B Bai, D Vanderwall, Y Li, X Wang, S Poudel… - Molecular …, 2021 - Springer
Mass spectrometry-based proteomics empowers deep profiling of proteome and protein
posttranslational modifications (PTMs) in Alzheimer's disease (AD). Here we review the …
posttranslational modifications (PTMs) in Alzheimer's disease (AD). Here we review the …