Strategies to Optimize Protein Expression in E. coli

DM Francis, R Page - Current protocols in protein science, 2010 - Wiley Online Library
Recombinant protein expression in Escherichia coli (E. coli) is simple, fast, inexpensive, and
robust, with the expressed protein comprising up to 50 percent of the total cellular protein …

Genetic regulation of nitrogen fixation in rhizobia

HM Fischer - Microbiological reviews, 1994 - journals.asm.org
This review presents a comparison between the complex genetic regulatory networks that
control nitrogen fixation in three representative rhizobial species, Rhizobium meliloti …

TRANSCRIPTIONAL CONTROL OF THE PSEUDOMONAS TOL PLASMID CATABOLIC OPERONS IS ACHIEVED THROUGH AN INTERPLAY OF HOST FACTORS …

JL Ramos, S Marqués, KN Timmis - Annual review of …, 1997 - annualreviews.org
▪ Abstract The xyl genes of Pseudomonas putida TOL plasmid that specify catabolism of
toluene and xylenes are organized in four transcriptional units: the upper-operon …

Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies

H Bach, Y Mazor, S Shaky, A Shoham-Lev… - Journal of molecular …, 2001 - Elsevier
Recombinant single-chain antibodies (scFvs) that are expressed in the cytoplasm of cells
are of considerable biotechnological and therapeutic potential. However, the reducing …

Effects of Temperature Stress on Bean-Nodulating Rhizobium Strains

J Michiels, C Verreth… - Applied and environmental …, 1994 - journals.asm.org
High soil temperatures in tropical areas limit nodulation and dinitrogen fixation by strains of
Rhizobium. Several heat-tolerant bean-nodulating Rhizobium strains have been isolated …

Single amino acid substitutions on the surface of Escherichia coli maltose‐binding protein can have a profound impact on the solubility of fusion proteins

JD Fox, RB Kapust, DS Waugh - Protein Science, 2001 - Wiley Online Library
Proteins are commonly fused to Escherichia coli maltose‐binding protein (MBP) to enhance
their yield and facilitate their purification. In addition, the stability and solubility of a …

The mosaic structure of the symbiotic plasmid of Rhizobium etliCFN42 and its relation to other symbiotic genome compartments

V González, P Bustos, MA Ramírez-Romero… - Genome biology, 2003 - Springer
Background Symbiotic bacteria known as rhizobia interact with the roots of legumes and
induce the formation of nitrogen-fixing nodules. In rhizobia, essential genes for symbiosis …

The oxygen-responsive NIFL-NIFA complex: a novel two-component regulatory system controlling nitrogenase synthesis in γ-Proteobacteria

R Dixon - Archives of microbiology, 1998 - Springer
The high energetic requirements for nitrogen fixation and the extreme oxygen sensitivity of
the nitrogenase enzyme impose physiological constraints on diazotrophy that necessitate …

Dual regulation of mucoidy in Pseudomonas aeruginosa and sigma factor antagonism

JC Boucher, MJ Schurr, V Deretic - Molecular microbiology, 2000 - Wiley Online Library
The conversion to mucoid, exopolysaccharide alginate‐overproducing phenotype in
Pseudomonas aeruginosa during chronic respiratory infections in cystic fibrosis patients …

The regulatory VirA protein of Agrobacterium tumefaciens does not function at elevated temperatures

S **, YN Song, WY Deng, MP Gordon… - Journal of …, 1993 - journals.asm.org
Previous studies have shown that Agrobacterium tumefaciens causes tumors on plants only
at temperatures below 32 degrees C, and virulence gene expression is specifically inhibited …