Synthetic chromosomes, genomes, viruses, and cells

JC Venter, JI Glass, CA Hutchison, S Vashee - Cell, 2022 - cell.com
Synthetic genomics is the construction of viruses, bacteria, and eukaryotic cells with
synthetic genomes. It involves two basic processes: synthesis of complete genomes or …

Bioremediation 3.0: engineering pollutant-removing bacteria in the times of systemic biology

P Dvořák, PI Nikel, J Damborský, V de Lorenzo - Biotechnology advances, 2017 - Elsevier
Elimination or mitigation of the toxic effects of chemical waste released to the environment
by industrial and urban activities relies largely on the catalytic activities of microorganisms …

Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions

Y Zhao, C Coelho, AL Hughes, L Lazar-Stefanita… - Cell, 2023 - cell.com
The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

Candidate phyla radiation, an underappreciated division of the human microbiome, and its impact on health and disease

S Naud, A Ibrahim, C Valles, M Maatouk… - Clinical Microbiology …, 2022 - Am Soc Microbiol
Candidate phyla radiation (CPR) is an emerging division of the bacterial domain within the
human microbiota. Still poorly known, these microorganisms were first described in the …

Establishing chromosomal design-build-test-learn through a synthetic chromosome and its combinatorial reconfiguration

JL Foo, S Kitano, AV Susanto, Z **, Y Lin, Z Luo… - Cell Genomics, 2023 - cell.com
Chromosome-level design-build-test-learn cycles (chrDBTLs) allow systematic
combinatorial reconfiguration of chromosomes with ease. Here, we established chrDBTL …

Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440

CW Johnson, PE Abraham, JG Linger, P Khanna… - Metabolic engineering …, 2017 - Elsevier
Carbon catabolite repression refers to the preference of microbes to metabolize certain
growth substrates over others in response to a variety of regulatory mechanisms. Such …

Bacillus subtilis, a swiss army knife in science and biotechnology

J Stülke, A Grüppen, M Bramkamp… - Journal of …, 2023 - Am Soc Microbiol
ABSTRACT Next to Escherichia coli, Bacillus subtilis is the most studied and best
understood organism that also serves as a model for many important pathogens. Due to its …

Toward a predictive understanding of Earth's microbiomes to address 21st century challenges

MJ Blaser, ZG Cardon, MK Cho, JL Dangl, TJ Donohue… - MBio, 2016 - Am Soc Microbiol
Microorganisms have shaped our planet and its inhabitants for over 3.5 billion years.
Humankind has had a profound influence on the biosphere, manifested as global climate …

Scaling up genetic circuit design for cellular computing: advances and prospects

Y **ang, N Dalchau, B Wang - Natural computing, 2018 - Springer
Synthetic biology aims to engineer and redesign biological systems for useful real-world
applications in biomanufacturing, biosensing and biotherapy following a typical design-build …

Discovery and significance of protein-protein interactions in health and disease

JF Greenblatt, BM Alberts, NJ Krogan - Cell, 2024 - cell.com
The identification of individual protein-protein interactions (PPIs) began more than 40 years
ago, using protein affinity chromatography and antibody co-immunoprecipitation. As new …