CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Structural basis for the activation of a compact CRISPR-Cas13 nuclease
The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and
transcriptional modulation owing to their high programmability and specificity. However, the …
transcriptional modulation owing to their high programmability and specificity. However, the …
Next-generation CRISPR technology for genome, epigenome and mitochondrial editing
CH Lau, QL Liang, H Zhu - Transgenic Research, 2024 - Springer
The application of rapidly growing CRISPR toolboxes and methods has great potential to
transform biomedical research. Here, we provide a snapshot of up-to-date CRISPR …
transform biomedical research. Here, we provide a snapshot of up-to-date CRISPR …
A strategy for Cas13 miniaturization based on the structure and AlphaFold
F Zhao, T Zhang, X Sun, X Zhang, L Chen… - Nature …, 2023 - nature.com
The small size of the Cas nuclease fused with various effector domains enables a broad
range of function. Although there are several ways of reducing the size of the Cas nuclease …
range of function. Although there are several ways of reducing the size of the Cas nuclease …
Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina
CRISPR-Cas13 nucleases are programmable RNA-targeting effectors that can silence gene
expression in a transient manner. Recent iterations of Cas13 nucleases are compact for …
expression in a transient manner. Recent iterations of Cas13 nucleases are compact for …
Insights gained from RNA editing targeted by the CRISPR-Cas13 family
L Liu, DS Pei - International Journal of Molecular Sciences, 2022 - mdpi.com
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein (Cas) systems, especially type II (Cas9) systems, have been widely developed for …
protein (Cas) systems, especially type II (Cas9) systems, have been widely developed for …
AAV-mediated gene therapies by miniature gene editing tools
X Kong, T Li, H Yang - Science China Life Sciences, 2024 - Springer
The advent of CRISPR-Cas has revolutionized precise gene editing. While pioneering
CRISPR nucleases like Cas9 and Cas12 generate targeted DNA double-strand breaks …
CRISPR nucleases like Cas9 and Cas12 generate targeted DNA double-strand breaks …
[PDF][PDF] CRISPR-Cas target recognition for sensing viral and cancer biomarkers
Nucleic acid-based diagnostics is a promising venue for detection of pathogens causing
infectious diseases and mutations related to cancer. However, this type of diagnostics still …
infectious diseases and mutations related to cancer. However, this type of diagnostics still …
Genome and transcriptome engineering by compact and versatile CRISPR-Cas systems
G Aquino-Jarquin - Drug Discovery Today, 2023 - Elsevier
Comparative genomics has enabled the discovery of tiny clustered regularly interspaced
short palindromic repeat (CRISPR) bacterial immune system effectors with enormous …
short palindromic repeat (CRISPR) bacterial immune system effectors with enormous …
Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme
Y Zhan, C Cao, A Li, H Mei, Y Liu - Genome Biology, 2023 - Springer
Background CRISPR-Cas13 is a newly emerging RNA knockdown technology that is
comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells …
comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells …