CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

The deubiquitylating enzymes (DUBs, also known as deubiquitylases or deubiquitinases)
maintain the dynamic state of the cellular ubiquitome by releasing conjugated ubiquitin from …

The vast majority of missense variants observed in the human genome are of unknown
clinical significance. We present AlphaMissense, an adaptation of AlphaFold fine-tuned on …

The accumulation of lipid peroxides is recognized as a determinant of the occurrence of
ferroptosis. However, the sensors and amplifying process of lipid peroxidation linked to …

Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …

Regulation of cytokine production in stimulated T cells can be disrupted in autoimmunity,
immunodeficiencies, and cancer. Systematic discovery of stimulation-dependent cytokine …

Functional genomics approaches can overcome limitations—such as the lack of
identification of robust targets and poor clinical efficacy—that hamper cancer drug …

Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are the primary
pathways for repairing DNA double-strand breaks (DSBs) during interphase, whereas …

Genetic variants that inactivate protein-coding genes are a powerful source of information
about the phenotypic consequences of gene disruption: genes that are crucial for the …

Genetic interactions mediate the emergence of phenotype from genotype, but technologies
for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we …