Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

M Tan, C Liao, L Liang, X Yi, Z Zhou… - Frontiers in cellular and …, 2022 - frontiersin.org
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people's lives. In
addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid …

Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

Gold nanoparticle-based plasmonic biosensors

E Ferrari - Biosensors, 2023 - mdpi.com
One of the emerging technologies in molecular diagnostics of the last two decades is the
use of gold nanoparticles (AuNPs) for biosensors. AuNPs can be functionalized with various …

Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

MK Nwe, N Jangpromma, L Taemaitree - Scientific Reports, 2024 - nature.com
Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform
assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is …

Conventional and novel diagnostic tools for the diagnosis of emerging SARS-CoV-2 variants

VP Chavda, DD Valu, PK Parikh, N Tiwari, AS Chhipa… - Vaccines, 2023 - mdpi.com
Accurate identification at an early stage of infection is critical for effective care of any
infectious disease. The “coronavirus disease 2019 (COVID-19)” outbreak, caused by the …

Loop-mediated isothermal amplification-integrated CRISPR methods for infectious disease diagnosis at point of care

D Yigci, N Atçeken, AK Yetisen, S Tasoglu - ACS omega, 2023 - ACS Publications
Infectious diseases continue to pose an imminent threat to global public health, leading to
high numbers of deaths every year and disproportionately impacting develo** countries …

Development of CRISPR/Cas13a-based assays for the diagnosis of schistosomiasis

SR MacGregor, DP McManus, H Sivakumaran… - …, 2023 - thelancet.com
Background Schistosomiasis is a disease that significantly impacts human health in the
develo** world. Effective diagnostics are urgently needed for improved control of this …

A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin

BJ Yee, NF Shafiqah, NF Mohd-Naim… - International Journal of …, 2023 - Elsevier
This study introduces CRISPR/Cas-based aptasensor for the highly sensitive and specific
detection of the antibiotic, ampicillin. Ampicillin (AMPI) is a commonly used antibiotic for …

Advances in point-of-care and molecular techniques to detect waterborne pathogens

M Khodaparast, D Sharley, S Marshall, T Beddoe - NPJ Clean Water, 2024 - nature.com
Rapid and precise detection of waterborne pathogens is critical for effective public health
management and environmental safety. Traditional methods for water quality monitoring …

Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

M Gavrilov, JYC Yang, RS Zou, W Ma, CY Lee… - Nature …, 2022 - nature.com
Abstract Polymerase Chain Reaction (PCR) is an essential method in molecular diagnostics
and life sciences. PCR requires thermal cycling for heating the DNA for strand separation …