[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Testing at scale during the COVID-19 pandemic

TR Mercer, M Salit - Nature Reviews Genetics, 2021 - nature.com
Assembly and publication of the severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) genome in January 2020 enabled the immediate development of tests to detect the …

Outbreak. info genomic reports: scalable and dynamic surveillance of SARS-CoV-2 variants and mutations

K Gangavarapu, AA Latif, JL Mullen, M Alkuzweny… - Nature …, 2023 - nature.com
In response to the emergence of SARS-CoV-2 variants of concern, the global scientific
community, through unprecedented effort, has sequenced and shared over 11 million …

Assignment of epidemiological lineages in an emerging pandemic using the pangolin tool

Á O'Toole, E Scher, A Underwood, B Jackson… - Virus …, 2021 - academic.oup.com
The response of the global virus genomics community to the severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) pandemic has been unprecedented, with significant …

The next phase of SARS-CoV-2 surveillance: real-time molecular epidemiology

BB Oude Munnink, N Worp, DF Nieuwenhuijse… - Nature medicine, 2021 - nature.com
The current coronavirus disease 2019 (COVID-19) pandemic is the first to apply whole-
genome sequencing near to real time, with over 2 million severe acute respiratory syndrome …

Clinical metagenomics

CY Chiu, SA Miller - Nature Reviews Genetics, 2019 - nature.com
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of
microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly …

Zika virus infection enhances future risk of severe dengue disease

LC Katzelnick, C Narvaez, S Arguello… - Science, 2020 - science.org
The Zika pandemic sparked intense interest in whether immune interactions among dengue
virus serotypes 1 to 4 (DENV1 to-4) extend to the closely related Zika virus (ZIKV). We …

Field-deployable viral diagnostics using CRISPR-Cas13

C Myhrvold, CA Freije, JS Gootenberg, OO Abudayyeh… - Science, 2018 - science.org
Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and
rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK …

Nextstrain: real-time tracking of pathogen evolution

J Hadfield, C Megill, SM Bell, J Huddleston… - …, 2018 - academic.oup.com
Understanding the spread and evolution of pathogens is important for effective public health
measures and surveillance. Nextstrain consists of a database of viral genomes, a …

Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection

T Charalampous, GL Kay, H Richardson, A Aydin… - Nature …, 2019 - nature.com
The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …