Following its invention 25 years ago, PCR has been adapted for numerous molecular
biology applications. Gene expression analysis by reverse-transcription quantitative PCR …

Abstract Background Quantitative PCR (qPCR) aims to measure the DNA or RNA
concentration in diagnostic and biological samples based on the quantification cycle (Cq) …

Background The 2-ΔΔCT method has been extensively used as a relative quantification
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …

Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA
transcripts, most analyses of qPCR data are still delegated to the software that comes with …

Background The analyses of amplification and melting curves have been shown to provide
valuable information on the quality of the individual reactions in quantitative PCR (qPCR) …

Nuclear lamin B1 (LB1) is a major structural component of the nucleus that appears to be
involved in the regulation of many nuclear functions. The results of this study demonstrate …

Sensory systems for detecting tactile stimuli have evolved from touch-sensing nerves in
invertebrates to complicated tactile end organs in mammals. Merkel discs are tactile end …

Context Telomeres are essential to preserve the integrity of the genome. Critically short
telomeres lead to replicative cell senescence and chromosomal instability and may thereby …

Stem cells are known to differentiate in response to the chemical and mechanical properties
of the substrates on which they are cultured. Thus, supramolecular biomaterials with tunable …

Background Recently, rapid improvements in technology and decrease in sequencing costs
have made RNA-Seq a widely used technique to quantify gene expression levels. Various …