A major goal of evolutionary genetics is to understand the genetic processes that give rise to
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …

Nearly all human complex traits and disease phenotypes exhibit some degree of sex
differences, including differences in prevalence, age of onset, severity or disease …

A flexible statistical framework is developed for the analysis of read counts from RNA-Seq
gene expression studies. It provides the ability to analyse complex experiments involving …

RNA sequencing (RNA-seq) has been rapidly adopted for the profiling of transcriptomes in
many areas of biology, including studies into gene regulation, development and disease. Of …

Background Finding genes that are differentially expressed between conditions is an
integral part of understanding the molecular basis of phenotypic variation. In the past …

RNA-Seq is a powerful tool for the study of alternative splicing and other forms of alternative
isoform expression. Understanding the regulation of these processes requires sensitive and …

Experimental variance is a major challenge when dealing with high-throughput sequencing
data. This variance has several sources: sampling replication, technical replication …

Abstract Changes in gene expression are thought to underlie many of the phenotypic
differences between species. However, large-scale analyses of gene expression evolution …

High-throughput RNA sequencing (RNA-seq) promises a comprehensive picture of the
transcriptome, allowing for the complete annotation and quantification of all genes and their …

Background Differential expression analysis based on “next-generation” sequencing
technologies is a fundamental means of studying RNA expression. We recently developed a …