Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Super-resolution techniques have begun to transform biological and biomedical research by
allowing researchers to observe structures well below the classic diffraction limit of light …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Counting molecules in complexes is challenging, even with super-resolution microscopy.
Here, we use the programmable and specific binding of dye-labeled DNA probes to count …

Because the degree of labeling (DOL) of cell-bound antibodies, often required in
quantitative fluorescence measurements, is largely unknown, we investigated the effect of …

Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

Understanding how building blocks of life contribute to physiology is greatly aided by protein
identification and cellular localization. The two main labeling approaches developed over …

Methods that fuse multiple localization microscopy images of a single structure can improve
signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity …

Single-molecule localization microscopy (SMLM) relies on the switching of fluorescent
molecules between a fluorescent and a dark state to achieve super resolution. This process …