Precision‐cut lung slices: a powerful ex vivo model to investigate respiratory infectious diseases

F Viana, CM O'Kane, GN Schroeder - Molecular Microbiology, 2022 - Wiley Online Library
Respiratory infections are a leading cause of mortality worldwide. Most of the research on
the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal …

[HTML][HTML] In vitro and ex vivo models in inhalation biopharmaceutical research—advances, challenges and future perspectives

MA Selo, JA Sake, KJ Kim, C Ehrhardt - Advanced Drug Delivery Reviews, 2021 - Elsevier
Oral inhalation results in pulmonary drug targeting and thereby reduces systemic side
effects, making it the preferred means of drug delivery for the treatment of respiratory …

Spray drying siRNA-lipid nanoparticles for dry powder pulmonary delivery

CM Zimmermann, D Baldassi, K Chan… - Journal of Controlled …, 2022 - Elsevier
While all the siRNA drugs on the market target the liver, the lungs offer a variety of currently
undruggable targets which could potentially be treated with RNA therapeutics. Hence, local …

Targeting genomic SARS-CoV-2 RNA with siRNAs allows efficient inhibition of viral replication and spread

S Ambike, CC Cheng, M Feuerherd… - Nucleic acids …, 2022 - academic.oup.com
A promising approach to tackle the severe acute respiratory syndrome coronavirus-2 (SARS-
CoV-2) could be small interfering (si) RNAs. So far it is unclear, which viral replication steps …

Ex vivo tissue perturbations coupled to single-cell RNA-seq reveal multilineage cell circuit dynamics in human lung fibrogenesis

NJ Lang, J Gote-Schniering… - Science translational …, 2023 - science.org
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing
mechanisms of regeneration and fibrogenesis directly in human tissue has been hampered …

Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage

C Beck, D Ramanujam, P Vaccarello… - Nature …, 2023 - nature.com
Recent studies of severe acute inflammatory lung disease including COVID-19 identify
macrophages to drive pulmonary hyperinflammation and long-term damage such as fibrosis …

Inhibition of SARS-CoV-2 replication in the lung with siRNA/VIPER polyplexes

D Baldassi, S Ambike, M Feuerherd, CC Cheng… - Journal of Controlled …, 2022 - Elsevier
SARS-CoV-2 has been the cause of a global pandemic since 2019 and remains a medical
urgency. siRNA-based therapies are a promising strategy to fight viral infections. By …

Applications and approaches for three-dimensional precision-cut lung slices. Disease modeling and drug discovery

HN Alsafadi, FE Uhl, RH Pineda, KE Bailey… - American journal of …, 2020 - atsjournals.org
Chronic lung diseases (CLDs), such as chronic obstructive pulmonary disease, interstitial
lung disease, and lung cancer, are among the leading causes of morbidity globally and …

Human-Based Advanced in vitro Approaches to Investigate Lung Fibrosis and Pulmonary Effects of COVID-19

M Kiener, N Roldan, C Machahua, A Sengupta… - Frontiers in …, 2021 - frontiersin.org
The coronavirus disease 2019 (COVID-19) pandemic has caused considerable socio-
economic burden, which fueled the development of treatment strategies and vaccines at an …

Post-COVID Syndrome: The Research Progress in the Treatment of Pulmonary sequelae after COVID-19 Infection

V Ruggiero, RP Aquino, P Del Gaudio, P Campiglia… - Pharmaceutics, 2022 - mdpi.com
Post-COVID syndrome or long COVID is defined as the persistence of symptoms after
confirmed SARS-CoV-2 infection, the pathogen responsible for coronavirus disease. The …