Immune checkpoint therapy (ICT) has dramatically altered clinical outcomes for cancer
patients and conferred durable clinical benefits, including cure in a subset of patients …

Advances in multiplexed imaging technologies have drastically improved our ability to
characterize healthy and diseased tissues at the single-cell level. Co-detection by indexing …

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that drives the
generation of myeloid cell subsets including neutrophils, monocytes, macrophages, and …

Systemic immune monitoring is a crucial clinical tool for disease early diagnosis, prognosis
and treatment planning by quantitative analysis of immune cells. However, conventional …

Cutaneous T cell lymphomas (CTCL) are rare but aggressive cancers without effective
treatments. While a subset of patients derive benefit from PD-1 blockade, there is a critically …

Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex
imaging technologies are limited in resolving subcellular biomolecular features. Expansion …

Understanding the mechanisms of HIV tissue persistence necessitates the ability to visualize
tissue microenvironments where infected cells reside; however, technological barriers limit …

High-content imaging is needed to catalog the variety of cellular phenotypes and
multicellular ecosystems present in metazoan tissues. We recently developed iterative …

Characterization of the tumor microenvironment through immunoprofiling has become an
essential resource for the understanding of the complex immune cell interactions and the …

Background Algorithmic cellular segmentation is an essential step for the quantitative
analysis of highly multiplexed tissue images. Current segmentation pipelines often require …