Emerging platforms for high-throughput enzymatic bioassays

F Shao, PW Lee, H Li, K Hsieh, TH Wang - Trends in biotechnology, 2023 - cell.com
Enzymes have essential roles in catalyzing biological reactions and maintaining metabolic
systems. Many in vitro enzymatic bioassays have been developed for use in industrial and …

A fully automated and integrated microfluidic system for efficient CTC detection and its application in hepatocellular carcinoma screening and prognosis

J Wang, Y Li, R Wang, C Han, S Xu, T You… - … Applied Materials & …, 2021 - ACS Publications
Analysis of circulating tumor cells (CTCs) is regarded as a useful diagnostic index to monitor
tumor development and guide precision medicine. Although the immunoassay is a common …

Functional analysis of single enzymes combining programmable molecular circuits with droplet-based microfluidics

G Gines, R Espada, A Dramé-Maigné… - Nature …, 2024 - nature.com
The analysis of proteins at the single-molecule level reveals heterogeneous behaviours that
are masked in ensemble-averaged techniques. The digital quantification of enzymes …

Facile and scalable tubing-free sample loading for droplet microfluidics

F Shao, K Hsieh, P Zhang, AM Kaushik, TH Wang - Scientific reports, 2022 - nature.com
Droplet microfluidics has in recent years found a wide range of analytical and bioanalytical
applications. In droplet microfluidics, the samples that are discretized into droplets within the …

Multiplex digital microRNA detection using cross-inhibitory DNA circuits

Y Rondelez, G Gines - ACS sensors, 2020 - ACS Publications
Ubiquitous post-transcriptional regulators in eukaryotes, microRNAs are currently emerging
as promising biomarkers of physiological and pathological processes. Multiplex and digital …

Digital PCR system development accelerator—A methodology to emulate dPCR results

H Zhang, Z Yan, X Wang, M Gaňová… - Sensors and Actuators B …, 2022 - Elsevier
The development of a digital polymerase chain reaction (dPCR) system typically begins with
an idea for the system configuration and chip layout, followed by design, fabrication, and …

Silicon chambers for enhanced incubation and imaging of microfluidic droplets

N Lobato-Dauzier, R Deteix, G Gines, A Baccouche… - Lab on a Chip, 2023 - pubs.rsc.org
Droplet microfluidics has become a powerful tool in life sciences, underlying digital assays,
single-cell sequencing or directed evolution, and it is making foray in physical sciences as …

[HTML][HTML] Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform

F Shao, H Li, K Hsieh, P Zhang, S Li… - Acta Pharmaceutica Sinica …, 2024 - Elsevier
Antimicrobial resistance (AMR) has become a global health crisis in need of novel solutions.
To this end, antibiotic combination therapies, which combine multiple antibiotics for …

Modular Droplet‐Based Fluidics for Large Volume Libraries of Individual Multiparametric Codes in Lab‐On‐Chip Systems

J Schütt, H Nhalil, J Fassbender, L Klein… - Advanced Sensor …, 2023 - Wiley Online Library
Droplet‐based lab‐on‐a‐chip systems offer vast possibilities in the manipulation, guidance,
tracking, and labeling of individual droplet‐based bioreactors. One of the targeted …

Silicon as a microfluidic material for imaging and incubation of droplets

N Lobato-Dauzier, R Deteix, G Gines, A Baccouche… - bioRxiv, 2022 - biorxiv.org
Droplet microfluidics has become a powerful tool in life sciences, underlying digital assays,
single-cell sequencing or directed evolution, and it is making foray in physical sciences as …