CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Gene editing and its applications in biomedicine
G Li, X Li, S Zhuang, L Wang, Y Zhu, Y Chen… - Science China Life …, 2022 - Springer
The steady progress in genome editing, especially genome editing based on the use of
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …
Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells
A Tomita, H Sasanuma, T Owa, Y Nakazawa… - Nature …, 2023 - nature.com
CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases.
However, its therapeutic applications are limited by unintended genomic alterations arising …
However, its therapeutic applications are limited by unintended genomic alterations arising …
Efficient prime editing in two-cell mouse embryos using PEmbryo
RP Kim-Yip, R McNulty, B Joyce, A Mollica… - Nature …, 2024 - nature.com
Using transient inhibition of DNA mismatch repair during a permissive stage of development,
we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local …
we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local …
Cohesin regulates homology search during recombinational DNA repair
Homologous recombination repairs DNA double-strand breaks (DSB) using an intact dsDNA
molecule as a template. It entails a homology search step, carried out along a conserved …
molecule as a template. It entails a homology search step, carried out along a conserved …
ON-target adverse events of CRISPR-Cas9 nuclease: more chaotic than expected
J Boutin, D Cappellen, J Rosier, S Amintas… - The CRISPR …, 2022 - liebertpub.com
CRISPR-Cas9 is a highly promising technology for clinical development. However, this
powerful tool can induce adverse genomic events. The off-target genotoxicity is well …
powerful tool can induce adverse genomic events. The off-target genotoxicity is well …
An update on precision genome editing by homology-directed repair in plants
J Chen, S Li, Y He, J Li, L **a - Plant Physiology, 2022 - academic.oup.com
Beneficial alleles derived from local landraces or related species, or even orthologs from
other plant species, are often caused by differences of one or several single-nucleotide …
other plant species, are often caused by differences of one or several single-nucleotide …
Cas9/Nickase-induced allelic conversion by homologous chromosome-templated repair in Drosophila somatic cells
Repair of double-strand breaks (DSBs) in somatic cells is primarily accomplished by error-
prone nonhomologous end joining and less frequently by precise homology-directed repair …
prone nonhomologous end joining and less frequently by precise homology-directed repair …
Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination
J Xu, L Zhao, S Peng, H Chu, R Liang… - Nucleic Acids …, 2021 - academic.oup.com
Homologous recombination (HR) is a primary DNA double-strand breaks (DSBs) repair
mechanism. The recombinases Rad51 and Dmc1 are highly conserved in the RecA family; …
mechanism. The recombinases Rad51 and Dmc1 are highly conserved in the RecA family; …
Limitations of gene editing assessments in human preimplantation embryos
D Liang, A Mikhalchenko, H Ma… - Nature …, 2023 - nature.com
Range of DNA repair in response to double-strand breaks induced in human
preimplantation embryos remains uncertain due to the complexity of analyzing single-or few …
preimplantation embryos remains uncertain due to the complexity of analyzing single-or few …