Next-generation sequencing technology: current trends and advancements

H Satam, K Joshi, U Mangrolia, S Waghoo, G Zaidi… - Biology, 2023 - mdpi.com
Simple Summary Next-generation sequencing (NGS) is a powerful tool used in genomics
research. NGS can sequence millions of DNA fragments at once, providing detailed …

A practical guide to amplicon and metagenomic analysis of microbiome data

YX Liu, Y Qin, T Chen, M Lu, X Qian, X Guo… - Protein & cell, 2021 - academic.oup.com
Advances in high-throughput sequencing (HTS) have fostered rapid developments in the
field of microbiome research, and massive microbiome datasets are now being generated …

MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data

Y Lu, G Zhou, J Ewald, Z Pang, T Shiri… - Nucleic Acids …, 2023 - academic.oup.com
Microbiome studies have become routine in biomedical, agricultural and environmental
sciences with diverse aims, including diversity profiling, functional characterization, and …

Greengenes2 unifies microbial data in a single reference tree

D McDonald, Y Jiang, M Balaban, K Cantrell… - Nature …, 2024 - nature.com
Studies using 16S rRNA and shotgun metagenomics typically yield different results, usually
attributed to PCR amplification biases. We introduce Greengenes2, a reference tree that …

Environmental stress destabilizes microbial networks

DJ Hernandez, AS David, ES Menges… - The ISME …, 2021 - academic.oup.com
Environmental stress is increasing worldwide, yet we lack a clear picture of how stress
disrupts the stability of microbial communities and the ecosystem services they provide …

Erosion reduces soil microbial diversity, network complexity and multifunctionality

L Qiu, Q Zhang, H Zhu, PB Reich, S Banerjee… - The ISME …, 2021 - academic.oup.com
While soil erosion drives land degradation, the impact of erosion on soil microbial
communities and multiple soil functions remains unclear. This hinders our ability to assess …

RESCRIPt: Reproducible sequence taxonomy reference database management

MS Robeson, DR O'Rourke, BD Kaehler… - PLoS computational …, 2021 - journals.plos.org
Nucleotide sequence and taxonomy reference databases are critical resources for
widespread applications including marker-gene and metagenome sequencing for …

Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major …

DW Waite, M Chuvochina, C Pelikan… - … of Systematic and …, 2020 - microbiologyresearch.org
The class Deltaproteobacteria comprises an ecologically and metabolically diverse group of
bacteria best known for dissimilatory sulphate reduction and predatory behaviour. Although …

A standardized archaeal taxonomy for the Genome Taxonomy Database

C Rinke, M Chuvochina, AJ Mussig, PA Chaumeil… - Nature …, 2021 - nature.com
The accrual of genomic data from both cultured and uncultured microorganisms provides
new opportunities to develop systematic taxonomies based on evolutionary relationships …

A complete domain-to-species taxonomy for Bacteria and Archaea

DH Parks, M Chuvochina, PA Chaumeil, C Rinke… - Nature …, 2020 - nature.com
Abstract The Genome Taxonomy Database is a phylogenetically consistent, genome-based
taxonomy that provides rank-normalized classifications for~ 150,000 bacterial and archaeal …