Retrovirus packaging cells

AD Miller - Human gene therapy, 1990 - liebertpub.com
Retroviral vectors promote the efficient transfer of genes into a variety of cell types from
many animal species. An important contribution to their utility was the development of …

Gene therapy for cancer: what have we done and where are we going?

JA Roth, RJ Cristiano - Journal of the National Cancer Institute, 1997 - academic.oup.com
Gene-based therapies for cancer in clinical trials include strategies that involve
augmentation of immunotherapeutic and chemotherapeutic approaches. These strategies …

Regeneration of the entire human epidermis using transgenic stem cells

T Hirsch, T Rothoeft, N Teig, JW Bauer, G Pellegrini… - Nature, 2017 - nature.com
Junctional epidermolysis bullosa (JEB) is a severe and often lethal genetic disease caused
by mutations in genes encoding the basement membrane component laminin-332 …

Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line

JP Morgenstern, H Land - Nucleic acids research, 1990 - academic.oup.com
We report the development of an advanced system for transfer and expression of exogenous
genes in mammalian cells based on Moloney murine leukemia virus (Mo MuLV). Extensive …

[HTML][HTML] A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers

JR Bischoff, L Anderson, Y Zhu, K Mossie, L Ng… - The EMBO …, 1998 - embopress.org
Genetic and biochemical studies in lower eukaryotes have identified several proteins that
ensure accurate segregation of chromosomes. These include the Drosophila aurora and …

[HTML][HTML] Improved retroviral vectors for gene transfer and expression

AD Miller, GJ Rosman - Biotechniques, 1989 - ncbi.nlm.nih.gov
We describe a set of murine retrovirus-based vectors that include unique cloning sites for
insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal …

The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses

RK Naviaux, E Costanzi, M Haas, IM Verma - Journal of virology, 1996 - Am Soc Microbiol
We describe the construction and characterization of retroviral vectors and packaging
plasmids that produce helper-free retrovirus with titers of 1 X 10 (6) to 5 X 10 (6) within 48 h …

Engraftable human neural stem cells respond to development cues, replace neurons, and express foreign genes

JD Flax, S Aurora, C Yang, C Simonin, AM Wills… - Nature …, 1998 - nature.com
Stable clones of neural stem cells (NSCs) have been isolated from the human fetal
telencephalon. These self-renewing clones give rise to all fundamental neural lineages in …

[HTML][HTML] Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1a but not Pbx1b

E Kroon, J Krosl, U Thorsteinsdottir, S Baban… - The EMBO …, 1998 - embopress.org
Abstract Hoxa9, Meis1 and Pbx1 encode homeodomaincontaining proteins implicated in
leukemic transformation in both mice and humans. Hoxa9, Meis1 and Pbx1 proteins have …

Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo.

G Sauvageau, U Thorsteinsdottir, CJ Eaves… - Genes & …, 1995 - genesdev.cshlp.org
Hox genes were first recognized for their role in embryonic development and may also play
important lineage-specific functions in a variety of somatic tissues including the …