The fungal vacuole is an extremely complex organelle that is involved in a wide variety of
functions. The vacuole not only carries out degradative processes, the role most often …

Publisher Summary The response of a fungus to osmotic stress involves the integrated
function of many components of cell metabolism. The dehydration stress is countered by an …

Interactions of toxic metals with fungi have long been of importance since metal toxicity still
is the basis of many fungicidal preparations while in an environmental context, accelerating …

All cells, including microbes, detect and respond to mechanical forces, of which osmotic
pressure is most ancient and universal. Channel proteins have evolved such that they can …

Internal Ca2+ release in yeast is triggered by hypertonic shock and mediated by a TRP
channel homologue Page 1 JCB © The Rockefeller University Press, 0021-9525/2002/1/29/6 …

A specific effect of Cu2+ eliciting selective changes in the permeability of intact
Saccharomyces cerevisiae cells is described. When 100 microM CuCl2 was added to a cell …

The molecular identification of ion channels in internal membranes has made scant
progress compared with the study of plasma membrane ion channels. We investigated a …

Studies of ion channels have for long been dominated by the animalcentric, if not
anthropocentric, view of physiology. The structures and activities of ion channels had …

Ion channels are proteins which catalyse rapid, passive, electrogenic uniport of ions through
pores spanning an otherwise poorly permeable lipid bilayer. Among other processes, fluxes …

Publisher Summary This chapter describes techniques used in the study of the vacuole and
focuses on the procedures used in the laboratories. The yeast vacuole is a large, dynamic …