Programming gene and engineered-cell therapies with synthetic biology
BACKGROUND Gene and engineered-cell therapies promise new treatment modalities for
incurable or difficult-to-treat diseases. First-generation gene and engineered-cell therapies …
incurable or difficult-to-treat diseases. First-generation gene and engineered-cell therapies …
Synthetic and systems biology for microbial production of commodity chemicals
The combination of synthetic and systems biology is a powerful framework to study
fundamental questions in biology and produce chemicals of immediate practical application …
fundamental questions in biology and produce chemicals of immediate practical application …
Cas9 gRNA engineering for genome editing, activation and repression
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were
able to control Cas9 nuclease activity and simultaneously perform genome editing and …
able to control Cas9 nuclease activity and simultaneously perform genome editing and …
Algorithmic programming of sequential logic and genetic circuits for recording biochemical concentration in a probiotic bacterium
Through the implementation of designable genetic circuits, engineered probiotic
microorganisms could be used as noninvasive diagnostic tools for the gastrointestinal tract …
microorganisms could be used as noninvasive diagnostic tools for the gastrointestinal tract …
Precision design of stable genetic circuits carried in highly‐insulated E. coli genomic landing pads
Genetic circuits have many applications, from guiding living therapeutics to ordering process
in a bioreactor, but to be useful they have to be genetically stable and not hinder the host …
in a bioreactor, but to be useful they have to be genetically stable and not hinder the host …
FlowCal: a user-friendly, open source software tool for automatically converting flow cytometry data from arbitrary to calibrated units
Flow cytometry is widely used to measure gene expression and other molecular biological
processes with single cell resolution via fluorescent probes. Flow cytometers output data in …
processes with single cell resolution via fluorescent probes. Flow cytometers output data in …
Customizable gene sensing and response without altering endogenous coding sequences
Synthetic biology aims to modify cellular behaviors by implementing genetic circuits that
respond to changes in cell state. Integrating genetic biosensors into endogenous gene …
respond to changes in cell state. Integrating genetic biosensors into endogenous gene …
Single strain control of microbial consortia
The scope of bioengineering is expanding from the creation of single strains to the design of
microbial communities, allowing for division-of-labour, specialised sub-populations and …
microbial communities, allowing for division-of-labour, specialised sub-populations and …
Standardization in synthetic biology: an engineering discipline coming of age
T Decoene, B De Paepe, J Maertens… - Critical reviews in …, 2018 - Taylor & Francis
Background: Lea** DNA read-and-write technologies, and extensive automation and
miniaturization are radically transforming the field of biological experimentation by providing …
miniaturization are radically transforming the field of biological experimentation by providing …
Systematic transfer of prokaryotic sensors and circuits to mammalian cells
Prokaryotic regulatory proteins respond to diverse signals and represent a rich resource for
building synthetic sensors and circuits. The TetR family contains> 105 members that use a …
building synthetic sensors and circuits. The TetR family contains> 105 members that use a …