Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
Enzyme engineering: Resha** the biocatalytic functions
Enzyme engineering is a powerful tool to fine‐tune the enzymes. It is a technique by which
the stability, activity, and specificity of the enzymes can be altered. The characteristic …
the stability, activity, and specificity of the enzymes can be altered. The characteristic …
Computational tools for designing and engineering enzymes
J Damborsky, J Brezovsky - Current opinion in chemical biology, 2014 - Elsevier
Highlights•Concepts of enzyme engineering.•Novelty in silico methods and
tools.•Bioinformatics for stability prediction.•Molecular modelling for mechanistic studies.•De …
tools.•Bioinformatics for stability prediction.•Molecular modelling for mechanistic studies.•De …
Gluten degrading enzymes for treatment of celiac disease
Celiac disease (CeD) affects about 1% of most world populations. It presents a wide
spectrum of clinical manifestations, ranging from minor symptoms to mild or severe …
spectrum of clinical manifestations, ranging from minor symptoms to mild or severe …
Importance of enzyme specificity and stability for the application of proteases in greener industrial processing-a review
Industries are in great need for process-apt proteases due to environmental concerns. But
the major bottlenecks in the use of enzymes in industrial processes are their lack of …
the major bottlenecks in the use of enzymes in industrial processes are their lack of …
Enzyme engineering: reaching the maximal catalytic efficiency peak
M Goldsmith, DS Tawfik - Current opinion in structural biology, 2017 - Elsevier
Highlights•Optimization plateaus are common when engineering enzymes for higher
catalytic efficiency.•These plateaus relate to fundamental properties of evolutionary fitness …
catalytic efficiency.•These plateaus relate to fundamental properties of evolutionary fitness …
Evolving therapy for celiac disease
S Yoosuf, GK Makharia - Frontiers in pediatrics, 2019 - frontiersin.org
Gluten is known to be the main triggering factor for celiac disease (CeD), an immune-
mediated disorder. CeD is therefore managed using a strict and lifelong gluten-free diet …
mediated disorder. CeD is therefore managed using a strict and lifelong gluten-free diet …
Enantioselective enzymes by computational design and in silico screening
Computational enzyme design holds great promise for providing new biocatalysts for
synthetic chemistry. A strategy to design small mutant libraries of complementary …
synthetic chemistry. A strategy to design small mutant libraries of complementary …
Tailoring enzyme microenvironment: State-of-the-art strategy to fulfill the quest for efficient bio-catalysis
Enzymes as green industrial biocatalysts have become a powerful norm that offers several
advantages over traditional catalytic agents with regard to process efficiency, reusability …
advantages over traditional catalytic agents with regard to process efficiency, reusability …
Novel approaches for enzymatic gluten degradation to create high-quality gluten-free products
Celiac disease (CD), a chronic enteropathy of the small intestine caused by ingestion of
gluten, is one of the most prevalent food hypersensitivities worldwide. The essential …
gluten, is one of the most prevalent food hypersensitivities worldwide. The essential …