Circular Engineered Sortase for Interrogating Histone H3 in Chromatin

SD Whedon, K Lee, ZA Wang, E Zahn… - Journal of the …, 2024 - ACS Publications
Reversible modification of the histone H3 N-terminal tail is critical in regulating the chromatin
structure, gene expression, and cell states, while its dysregulation contributes to disease …

Efficient Chemical Synthesis of Multi-Monoubiquitylated and Diubiquitylated Histones by the α-Halogen Ketone-Mediated Strategy

S Peng, X Liu, C Lu, H Wang, X Liu, Q Gong… - Bioconjugate …, 2024 - ACS Publications
The chemical synthesis of homogeneously ubiquitylated histones is a powerful approach to
decipher histone ubiquitylation-dependent epigenetic regulation. Among the various …

RAD18-catalysed formation of ubiquitination intermediate mimic of proliferating cell nuclear antigen PCNA

L Zhang, Z Deng, Y Du, Z Xu, T Zhang, Z Tong… - Bioorganic & Medicinal …, 2025 - Elsevier
Abstract The 2-((2-chloroethyl) amino) ethane-1-thiol (CAET)-based chemical trap**
strategy is a practical tool for mechanistic studies of E3-catalysed ubiquitination. However …

Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents

H Cai, X Wu, J Mao, Z Tong, D Yan, Y Weng… - Bioorganic …, 2024 - Elsevier
Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide
range of cellular processes. The integrative identification of Ub-interacting proteins (readers) …