Molecular basis for redox control by the human cystine/glutamate antiporter system xc−
Cysteine plays an essential role in cellular redox homoeostasis as a key constituent of the
tripeptide glutathione (GSH). A rate limiting step in cellular GSH synthesis is the availability …
tripeptide glutathione (GSH). A rate limiting step in cellular GSH synthesis is the availability …
The human LAT1–4F2hc (SLC7A5–SLC3A2) transporter complex: Physiological and pathophysiological implications
J Kahlhofer, D Teis - Basic & Clinical Pharmacology & …, 2023 - Wiley Online Library
LAT1 and 4F2hc form a heterodimeric membrane protein complex, which functions as one of
the best characterized amino acid transporters. Since LAT1–4F2hc is required for the …
the best characterized amino acid transporters. Since LAT1–4F2hc is required for the …
Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris
Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia
pastoris) has been developed into the most popular yeast cell factory for the production of …
pastoris) has been developed into the most popular yeast cell factory for the production of …
Structural basis for substrate specificity of heteromeric transporters of neutral amino acids
CF Rodriguez, P Escudero-Bravo… - Proceedings of the …, 2021 - National Acad Sciences
Despite having similar structures, each member of the heteromeric amino acid transporter
(HAT) family shows exquisite preference for the exchange of certain amino acids. Substrate …
(HAT) family shows exquisite preference for the exchange of certain amino acids. Substrate …
Druggable Metabolic Vulnerabilities are exposed and masked during progression to castration resistant prostate cancer
SYC Choi, CF Ribeiro, Y Wang, M Loda, SR Plymate… - Biomolecules, 2022 - mdpi.com
There is an urgent need for exploring new actionable targets other than androgen receptor
to improve outcome from lethal castration-resistant prostate cancer. Tumor metabolism has …
to improve outcome from lethal castration-resistant prostate cancer. Tumor metabolism has …
The heavy chain 4F2hc modulates the substrate affinity and specificity of the light chains LAT1 and LAT2
S Kantipudi, JM Jeckelmann, Z Ucurum… - International journal of …, 2020 - mdpi.com
The human L-type amino acid transporters LAT1 and LAT2 mediate the transport of amino
acids and amino acid derivatives across plasma membranes in a sodium-independent …
acids and amino acid derivatives across plasma membranes in a sodium-independent …
Structure of the human heterodimeric transporter 4F2hc-LAT2 in complex with Anticalin, an alternative binding protein for applications in single-particle cryo-EM
Cryo-EM structure determination of relatively small and flexible membrane proteins at high
resolution is challenging. Increasing the size and structural features by binding of high …
resolution is challenging. Increasing the size and structural features by binding of high …
Characterization of substrates and inhibitors of the human heterodimeric transporter 4F2hc-LAT1 using purified protein and the scintillation proximity radioligand …
S Kantipudi, D Harder, D Fotiadis - Frontiers in physiology, 2023 - frontiersin.org
Amino acids have diverse and essential roles in many cellular functions such as in protein
synthesis, metabolism and as precursors of different hormones. Translocation of amino …
synthesis, metabolism and as precursors of different hormones. Translocation of amino …
Pichia pastoris and the Recombinant Human Heterodimeric Amino Acid Transporter 4F2hc-LAT1: From Clone Selection to Pure Protein
S Kantipudi, D Harder, S Bonetti, D Fotiadis… - Methods and …, 2021 - mdpi.com
Heterodimeric amino acid transporters (HATs) are protein complexes composed of two
subunits, a heavy and a light subunit belonging to the solute carrier (SLC) families SLC3 …
subunits, a heavy and a light subunit belonging to the solute carrier (SLC) families SLC3 …
[PDF][PDF] Jean‑Marc Jeckelmann1, 2Ε, Thomas Lemmin1, Martin Schlapschy3, Arne Skerra3 &
D Fotiadis - Scientific Reports, 2022 - academia.edu
Cryo‑EM structure determination of relatively small and flexible membrane proteins at high
resolution is challenging. Increasing the size and structural features by binding of high …
resolution is challenging. Increasing the size and structural features by binding of high …