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Probing neural codes with two-photon holographic optogenetics
Optogenetics ushered in a revolution in how neuroscientists interrogate brain function.
Because of technical limitations, the majority of optogenetic studies have used low spatial …
Because of technical limitations, the majority of optogenetic studies have used low spatial …
Fluorescence imaging of large-scale neural ensemble dynamics
Recent progress in fluorescence imaging allows neuroscientists to observe the dynamics of
thousands of individual neurons, identified genetically or by their connectivity, across …
thousands of individual neurons, identified genetically or by their connectivity, across …
[HTML][HTML] Large-scale two-photon calcium imaging in freely moving mice
We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution,
multiplane calcium imaging of over 1,000 neurons at a time in freely moving mice. With a …
multiplane calcium imaging of over 1,000 neurons at a time in freely moving mice. With a …
[HTML][HTML] Tracking the same neurons across multiple days in Ca2+ imaging data
Ca 2+ imaging techniques permit time-lapse recordings of neuronal activity from large
populations over weeks. However, without identifying the same neurons across imaging …
populations over weeks. However, without identifying the same neurons across imaging …
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
We have developed a miniature two-photon microscope equipped with an axial scanning
mechanism and a long-working-distance miniature objective to enable multi-plane imaging …
mechanism and a long-working-distance miniature objective to enable multi-plane imaging …
Circuit investigations with open-source miniaturized microscopes: past, present and future
The ability to simultaneously image the spatiotemporal activity signatures from many
neurons during unrestrained vertebrate behaviors has become possible through the …
neurons during unrestrained vertebrate behaviors has become possible through the …
Imaging calcium in neurons
Calcium ions generate versatile intracellular signals that control key functions in all types of
neurons. Imaging calcium in neurons is particularly important because calcium signals exert …
neurons. Imaging calcium in neurons is particularly important because calcium signals exert …
Miniaturized integration of a fluorescence microscope
The light microscope is traditionally an instrument of substantial size and expense. Its
miniaturized integration would enable many new applications based on mass-producible …
miniaturized integration would enable many new applications based on mass-producible …
Imprinting and recalling cortical ensembles
Neuronal ensembles are coactive groups of neurons that may represent building blocks of
cortical circuits. These ensembles could be formed by Hebbian plasticity, whereby synapses …
cortical circuits. These ensembles could be formed by Hebbian plasticity, whereby synapses …
Modulation of visual responses by behavioral state in mouse visual cortex
Studies of visual processing in rodents have conventionally been performed on
anesthetized animals, precluding examination of the effects of behavior on visually evoked …
anesthetized animals, precluding examination of the effects of behavior on visually evoked …