The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
Modifications in organic acid profiles during fruit development and ripening: correlation or causation?
The pivotal role of phytohormones during fruit development and ripening is considered
established knowledge in plant biology. Perhaps less well-known is the growing body of …
established knowledge in plant biology. Perhaps less well-known is the growing body of …
CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
JP Concordet, M Haeussler - Nucleic acids research, 2018 - academic.oup.com
CRISPOR. org is a web tool for genome editing experiments with the CRISPR–Cas9 system.
It finds guide RNAs in an input sequence and ranks them according to different scores that …
It finds guide RNAs in an input sequence and ranks them according to different scores that …
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Broad use of CRISPR–Cas12a (formerly Cpf1) nucleases has been hindered by the
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
Abstract The CRISPR-Cas12a RNA-guided complexes have tremendous potential for
nucleic acid detection but are limited to the picomolar detection limit without an amplification …
nucleic acid detection but are limited to the picomolar detection limit without an amplification …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
CRISPR-Cas tools and their application in genetic engineering of human stem cells and organoids
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
[HTML][HTML] CRISPR-Cas13d induces efficient mRNA knockdown in animal embryos
G Kushawah, L Hernandez-Huertas, JAN Del Prado… - Developmental Cell, 2020 - cell.com
Early embryonic development is driven exclusively by maternal gene products deposited
into the oocyte. Although critical in establishing early developmental programs, maternal …
into the oocyte. Although critical in establishing early developmental programs, maternal …
Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools
J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …