The AID/APOBEC family of enzymes in higher vertebrates converts cytosines in DNA or RNA
to uracil. They play a role in antibody maturation and innate immunity against viruses, and …

Metabolic burden as a consequence of overexpression of target gene in a recombinant
strain of E. coli 1727 has been analyzed with respect to maintenance energy coefficient (m) …

The bacteriophage T4 dam gene, encoding the Dam DNA [N 6-adenine] methyltransferase
(MTase), has been subcloned into the plasmid expression vector, pJW2. In this construct …

Revised Manuscript Received June 30, 1992 abstract: The type-II DNA-(cytosine-5)-
methyltransferase M. Hhal was overexpressed in Escherichia coli and purified to apparent …

(AdoMet), producing S-adenosyl-l-homocysteine (AdoHcy) and methylated DNA. The methyl
group acceptor atom is either an exocyclic amino nitrogen (N6-Ade or N4-Cyt) or a ring …

DNA methyltransferases (MTases) are sequence‐specific enzymes which transfer a methyl
group from S‐adenosyl‐l‐methionine (AdoMet) to the amino group of either cytosine or …

Overexpression of target gene (MspI methylase) in recombinant Escherichia coli has been
studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene …

A kinetic analysis of MspI DNA methyltransferase (M. MspI) is presented. The enzyme
catalyzes methylation of λ-DNA, a 50-kilobase pair linear molecule with multiple M. MspI …

The Hpa ll methylase (M. Hpa ll) recognizes the sequence CCGG and methylates the inner
cytosine residue. The Mspl methylase (Mspl) recognizes the same sequence but methytates …

Restriction‐modification systems have yielded enzymes central to biotechnology, but their
natural roles appear to have fundamental importance in the genetic ecology of the …