Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity

A Czarna, J Plewka, L Kresik, A Matsuda, A Karim… - Structure, 2022 - cell.com
During RNA replication, coronaviruses require proofreading to maintain the integrity of their
large genomes. Nsp14 associates with viral polymerase complex to excise the mismatched …

Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering

W Knecht, SZ Fisher, J Lou, C Sele, S Ma… - International Journal of …, 2023 - mdpi.com
The β-coronavirus family, encompassing Severe Acute Respiratory Syndrome Coronavirus
2 (SARS-CoV-2), Severe Acute Respiratory Syndrome Coronavirus (SARS), and Middle …

The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity

SL Grimes, BE Heaton, ML Anderson, K Burke… - Journal of …, 2025 - journals.asm.org
Coronaviruses (CoVs) encode non-structural proteins (nsp's) 1–16, which assemble to form
replication-transcription complexes that function in viral RNA synthesis. All CoVs encode a …

Substrate specificity and kinetics of RNA hydrolysis by SARS-CoV-2 NSP10/14 exonuclease

TL Dangerfield, KA Johnson - ACS Bio & Med Chem Au, 2022 - ACS Publications
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the virus that causes
COVID-19, continues to evolve resistance to vaccines and existing antiviral therapies at an …

A post-assembly conformational change makes the SARS-CoV-2 polymerase elongation-competent

M Klein, A Das, SC BERA, TK Anderson, D Kocincova… - bioRxiv, 2025 - biorxiv.org
Coronaviruses (CoV) encode sixteen non-structural proteins (nsps), most of which form the
replication-transcription complex (RTC). The RTC contains a core composed of one nsp12 …

Oligomeric state of β-coronavirus non-structural protein 10 stimulators studied by OmniSEC and Small Angle X-ray Scattering

W Knecht, SZ Fisher, J Lou, C Sele, S Ma… - bioRxiv, 2023 - biorxiv.org
Members of the β-coronavirus family such as SARS-CoV-2, SARS, and MERS have caused
pandemics over the last 20 years. Future pandemics are likely and studying the coronavirus …

[HTML][HTML] Coronavirus 2′-O-methyltransferase: A promising therapeutic target

C Schindewolf, VD Menachery - Virus Research, 2023 - Elsevier
Coronaviruses (CoVs) have been the source of multiple epidemics and a global pandemic
since the start of century, and there is an urgent need to understand CoV biology and …