The past decades of advancements in NMR have made it a very powerful tool for metabolic
research. Despite its limitations in sensitivity relative to mass spectrometric techniques, NMR …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Cancer cells consume large quantities of glucose and primarily use glycolysis for ATP
production, even in the presence of adequate oxygen,. This metabolic signature (aerobic …

Tumor cell proliferation requires rapid synthesis of macromolecules including lipids,
proteins, and nucleotides. Many tumor cells exhibit rapid glucose consumption, with most of …

Metabolic engineering is the directed improvement of cellular properties through the
modification of specific biochemical reactions or the introduction of new ones, with the use of …

TAp73 is a structural homologue of the pre-eminent tumour suppressor p53. However,
unlike p53, TAp73 is rarely mutated, and instead is frequently overexpressed in human …

Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high
glucose and glutamine uptake combined with a high rate of lactate and non-essential amino …

To determine the in vivo fluxes of the central metabolism we have developed a
comprehensive approach exclusively based on the fundamental enzyme reactions known to …

Traditionally, the intracellular fluxes of complex metabolic networks were quantified by
isotopic-tracer experiments, but, owing to practical limitations,'metabolic-flux balancing'is …

The estimation of the intracellular fluxes of mammalian cells using only the mass balances of
the relevant metabolites is not possible because the set of linear equations defined by these …