Material-binding peptides (MBPs) have emerged as a diverse and innovation-enabling class
of peptides in applications such as plant-/human health, immobilization of catalysts …

Directed evolution has advanced into a standard industrial “tool” to tailor naturally occurring
proteins for a variety of biotechnological applications, thus enabling product valorization and …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Iterative saturation mutagenesis (ISM) is a new and efficient method for the directed
evolution of functional enzymes. It reduces the necessary molecular biological work and the …

Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …

Methods to enhance random mutagenesis in cells offer advantages over in vitro
mutagenesis, but current in vivo methods suffer from a lack of control, genomic instability …

Numerous studies have noted that the evolution of new enzymatic specificities is
accompanied by loss of the protein's thermodynamic stability (ΔΔ G), thus suggesting a …

New technologies have recently enabled saturation mutagenesis and functional analysis of
nearly all possible variants of regulatory elements or proteins of interest in single …

Random mutagenesis methods only partially cover the mutational space and are
constrained by DNA synthesis length limitations. Here we demonstrate programmed allelic …

Directed evolution and rational design are powerful strategies in protein engineering to tailor
enzyme properties to meet the demands in academia and industry. Traditional approaches …