The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …

The free energy landscape concept that describes enzymes as an ensemble of differently
populated conformational sub-states in dynamic equilibrium is key for evaluating enzyme …

Amidases are robust biocatalysts for the industrial synthesis of carboxylic acids. Amidase-
catalyzed hydrolysis of 2-chloronicotinamide is regarded as a promising route for 2 …

Enzymes can produce high-quality food with low pollution, high function, high acceptability,
and medical aid. However, most enzymes, in their native form, do not meet the industrial …

With the development of artificial intelligence (AI), tailoring methods for enzyme engineering
have been widely expanded. Additional protocols based on optimized network models have …

Threonine deaminase (TD, EC 4.3. 1.19) mediates α, β-elimination (deamination), which has
untapped potential in the synthesis of unnatural α-keto acids. However, the narrow substrate …

Directed evolution of enzymes for the asymmetric reduction of prochiral ketones to produce
enantio‐pure secondary alcohols is particularly attractive in organic synthesis. Loops …

4-Hydroxyphenylacetate-3-hydroxylase (4HPA3H; EC 1.14. 14.9) is a heterodimeric flavin-
dependent monooxygenase complex that catalyzes the ortho-hydroxylation of resveratrol to …

Lipases are among the most applied biocatalysts in organic synthesis to catalyze the kinetic
resolution of a wide range of racemic substrates to yield optically pure compounds. Due to …

Mechanism‐based inactivation of l‐aspartate‐α‐decarboxylase (PanD), which leads to
irreversible modification of active site, is a major challenge in the efficient production of β …