RNA molecules start assembling into ribonucleoprotein (RNP) complexes during
transcription. Dynamic RNP assembly, largely directed by cis-acting elements on the RNA …

ABSTRACT Introduction: Cell penetrating peptides (CPPs) known as protein translocation
domains (PTD), membrane translocating sequences (MTS), or Trojan peptides (TP) are able …

Transcriptome profiling of single cells resident in their natural microenvironment depends
upon RNA capture methods that are both noninvasive and spatially precise. We engineered …

Riboswitches are intricate, metabolite-binding RNA structures found in the non-coding
regions of mRNA. Allosteric changes in the riboswitch that are induced by metabolite …

Cell penetrating peptides, generally categorized as amphipathic or cationic depending on
their sequence, are increasingly drawing attention as a non-invasive delivery technology for …

High molecular weight biomolecules are becoming more and more important in the
development of new therapeutic drugs. However, the hydrophilic nature of such molecules …

Axonal protein synthesis and degradation are rapidly regulated by extrinsic signals during
neural wiring, but the full landscape of proteomic changes remains unknown due to …

RNA binding proteins (RBPs) are key players in the regulation of gene expression. In this
chapter we discuss the main protein–RNA recognition modes used by RBPs in order to …

Immunoprecipitation of mRNA-protein complexes is a method that can be used to study RNA
binding protein (RBP)–RNA interactions. In this protocol, an antibody targeting an RBP of …

The importance of RNA-protein interactions in controlling mRNA regulation and non-coding
RNA function is increasingly appreciated. A variety of methods exist to comprehensively …