Gene regulatory network inference in the era of single-cell multi-omics

P Badia-i-Mompel, L Wessels, S Müller-Dott… - Nature Reviews …, 2023 - nature.com
The interplay between chromatin, transcription factors and genes generates complex
regulatory circuits that can be represented as gene regulatory networks (GRNs). The study …

The spatial organization of transcriptional control

A Hafner, A Boettiger - Nature Reviews Genetics, 2023 - nature.com
In animals, the sequences for controlling gene expression do not concentrate just at the
transcription start site of genes, but are frequently thousands to millions of base pairs distal …

Nuclear compartmentalization as a mechanism of quantitative control of gene expression

P Bhat, D Honson, M Guttman - Nature Reviews Molecular Cell Biology, 2021 - nature.com
Gene regulation requires the dynamic coordination of hundreds of regulatory factors at
precise genomic and RNA targets. Although many regulatory factors have specific affinity for …

Enhancer–promoter interactions and transcription are largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1

THS Hsieh, C Cattoglio, E Slobodyanyuk, AS Hansen… - Nature …, 2022 - nature.com
It remains unclear why acute depletion of CTCF (CCCTC-binding factor) and cohesin only
marginally affects expression of most genes despite substantially perturbing three …

Advances and opportunities in RNA structure experimental determination and computational modeling

J Zhang, Y Fei, L Sun, QC Zhang - Nature methods, 2022 - nature.com
Beyond transferring genetic information, RNAs are molecules with diverse functions that
include catalyzing biochemical reactions and regulating gene expression. Most of these …

[HTML][HTML] Spatial and temporal organization of the genome: Current state and future aims of the 4D nucleome project

J Dekker, F Alber, S Aufmkolk, BJ Beliveau… - Molecular cell, 2023 - cell.com
The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …

Mechanisms of Polycomb group protein function in cancer

V Parreno, AM Martinez, G Cavalli - Cell research, 2022 - nature.com
Cancer arises from a multitude of disorders resulting in loss of differentiation and a stem cell-
like phenotype characterized by uncontrolled growth. Polycomb Group (PcG) proteins are …

New insights into genome folding by loop extrusion from inducible degron technologies

E de Wit, EP Nora - Nature Reviews Genetics, 2023 - nature.com
Chromatin folds into dynamic loops that often span hundreds of kilobases and physically
wire distant loci together for gene regulation. These loops are continuously created …

Multiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization

J Zagelbaum, A Schooley, J Zhao… - Nature Structural & …, 2023 - nature.com
Nuclear actin-based movements have been shown to orchestrate clustering of DNA double-
strand breaks (DSBs) into homology-directed repair domains. Here we describe multiscale …

Cell-type-specific prediction of 3D chromatin organization enables high-throughput in silico genetic screening

J Tan, N Shenker-Tauris, J Rodriguez-Hernaez… - Nature …, 2023 - nature.com
Investigating how chromatin organization determines cell-type-specific gene expression
remains challenging. Experimental methods for measuring three-dimensional chromatin …