Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …

Environmental DNA (eDNA): Powerful technique for biodiversity conservation

A Sahu, N Kumar, CP Singh, M Singh - Journal for Nature Conservation, 2023 - Elsevier
Environmental DNA metabarcoding is a non-invasive method for discovering and identifying
rare and endangered species in a variety of ecosystems, including aquatic environments …

Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation

D Pont, M Rocle, A Valentini, R Civade, P Jean… - Scientific reports, 2018 - nature.com
Despite the ecological and societal importance of large rivers, fish sampling remains costly
and limited to specific habitats (eg, river banks). Using an eDNA metabarcoding approach …

Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment

M Stat, MJ Huggett, R Bernasconi, JD DiBattista… - Scientific reports, 2017 - nature.com
Effective marine management requires comprehensive data on the status of marine
biodiversity. However, efficient methods that can document biodiversity in our oceans are …

Understanding PCR processes to draw meaningful conclusions from environmental DNA studies

RP Kelly, AO Shelton, R Gallego - Scientific reports, 2019 - nature.com
As environmental DNA (eDNA) studies have grown in popularity for use in ecological
applications, it has become clear that their results differ in significant ways from those of …

An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data

LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …