Conservation in vitro of rare and threatened ferns—case studies of biodiversity hotspot and island species

H Barnicoat, R Cripps, J Kendon, V Sarasan - In Vitro Cellular & …, 2011 - Springer
The importance of in vitro tools to complement other ex situ methods for saving plants from
extinction is more relevant than ever before. More than 50% of the world's plant species are …

Structural mechanics of seed deterioration: standing the test of time

C Walters, D Ballesteros, VA Vertucci - Plant Science, 2010 - Elsevier
Seeds die inevitably but unexpectedly during storage and current understanding of seed
quality and storage conditions do not allow reliable means to predict or prevent this critical …

Fern conservation: spore, gametophyte, and sporophyte ex situ storage, in vitro culture, and cryopreservation

D Ballesteros, VC Pence - Current advances in fern research, 2018 - Springer
Many species of ferns and lycophytes are threatened by habitat destruction, over-collecting,
invasive species, and climate change, and plans to secure their in situ and ex situ …

Does oxygen affect ageing mechanisms of Pinus densiflora seeds? A matter of cytoplasmic physical state

D Gerna, D Ballesteros, E Arc, W Stöggl… - Journal of …, 2022 - academic.oup.com
During desiccation, the cytoplasm of orthodox seeds solidifies into an intracellular glass with
highly restricted diffusion and molecular mobility. Temperature and water content govern …

Detailed characterization of mechanical properties and molecular mobility within dry seed glasses: relevance to the physiology of dry biological systems

D Ballesteros, C Walters - The Plant Journal, 2011 - Wiley Online Library
Slow movement of molecules in glassy matrices controls the kinetics of chemical and
physical reactions in dry seeds. Variation in physiological activity among seeds suggests …

Role of relative humidity, temperature, and water status in dormancy alleviation of sunflower seeds during dry after-ripening

J Bazin, D Batlla, S Dussert… - Journal of …, 2011 - academic.oup.com
The effect of various combinations of temperature and relative humidity on dormancy
alleviation of sunflower seeds during dry after-ripening was investigated. The rate of …

Desiccation tolerance in ferns: from the unicellular spore to the multi-tissular sporophyte

M López-Pozo, B Fernández-Marín… - Current advances in fern …, 2018 - Springer
Desiccation tolerance (DT) represents an ecophysiological strategy probably developed
during land conquest by primitive plants that have been progressively lost in photosynthetic …

Effects of temperature and desiccation on ex situ conservation of nongreen fern spores

D Ballesteros, E Estrelles, C Walters… - American journal of …, 2012 - Wiley Online Library
• Premise of the study: Fern spores are unicellular and haploid, making them a potential
model system to study factors that regulate lifespan and mechanisms of aging. Aging rates of …

Variation of desiccation tolerance and longevity in fern spores

D Ballesteros, LM Hill, C Walters - Journal of Plant Physiology, 2017 - Elsevier
This work contributes to the understanding of plant cell responses to extreme water stress
when it is applied at different intensity and duration. Fern spores are used to explore survival …

Effect of storage temperature on green spore longevity for the ferns Equisetum ramosissimum and Osmunda regalis

D Ballesteros, E Estrelles, C Walters, AM Ibars - CryoLetters, 2011 - ingentaconnect.com
Green spores of ferns lose viability quickly, and need specialized treatment for long-term
conservation in germplasm banks. Dry storage at different temperatures was studied in …