Highlights•Comprehensive review of advances in microfluidic 3D cell culture since
2012.•Increased physiological relevance by precise control of cellular microenvironment.• …

Using polymer materials to fabricate microfluidic devices provides simple, cost effective, and
disposal advantages for both lab-on-a-chip (LOC) devices and micro total analysis systems …

Improved model systems to predict drug efficacy, interactions, and drug-induced kidney
injury (DIKI) are crucially needed in drug development. Organ-on-a-chip technology is a …

Organ-on-a-chip technology tries to mimic the complexity of native tissues in vitro. Important
progress has recently been made in using this technology to study the gut with and without …

Microfluidics permit the automated manipulation of fluids at the microscale with high
throughput and spatiotemporal precision, enabling the generation of large, multidimensional …

Aflatoxins, produced mainly by filamentous fungi Aspergillus flavus and Aspergillus
parasiticus, are one of the most carcinogenic compounds that have adverse health effects …

Single cell analysis has become of great interest with unprecedented capabilities for the
systematic investigation of cell-to-cell variation in large populations. Rapid and multi …

The organs-on-a-chip technology has shown strong promise in mimicking the complexity of
native tissues in vitro and ex vivo, and recently significant advances have been made in …

Droplet-based microfluidics is a colloidal and interfacial system that has rapidly progressed
in the past decade because of the advantages of low fabrication costs, small sample …

The human gut is responsible for food digestion and absorption. Recently, growing evidence
has shown its vital role in the proper functioning of other organs. Advances in microfluidic …