Highlights•Directed evolution is a protein engineering method mimicking natural
evolution.•Screening is the most time-and labor-intensive step in biocatalyst …

Biocatalytic alkylations are important reactions to obtain chemo‐, regio‐and
stereoselectively alkylated compounds. This can be achieved using S‐adenosyl‐l …

Next-generation sequencing doubles genomic databases every 2.5 years. The
accumulation of sequence data provides a unique opportunity to identify interesting …

Halide methyltransferases (HMTs) enable the enzymatic synthesis of S‐adenosyl‐l‐
methionine (SAM) from S‐adenosyl‐l‐homocysteine (SAH) and methyl iodide …

Enzymes play a crucial role in sustainable industrial applications, with their optimization
posing a formidable challenge due to the intricate interplay among residues. Computational …

Haloalkane dehalogenases and epoxide hydrolases are phylogenetically related and
structurally homologous enzymes that use nucleophilic aspartate residues for an SN2 attack …

Chloromethane (CH3Cl) is the most abundant halogenated volatile organic compound in
the atmosphere and contributes to stratospheric ozone depletion. CH3Cl has mainly natural …

Abstract Biokatalytische Alkylierungen sind wichtige Reaktionen, um chemo‐, regio‐und
stereoselektiv alkylierte Verbindungen zu erhalten. Dies kann mit S‐Adenosyl‐l‐methionin …

Engineering enzymes to catalyze non-native substrates is critical for chemical synthesis. A
significant challenge is to create a tool specialized in shifting an enzyme's activity toward a …

Halides are substrates and products of a number of biotechnologically important enzymes
like dehalogenases, halide methyltransferases, and halogenases. Therefore, the …