Protein is a major component of all biological evidence, often the matrix that embeds other
biomolecules such as polynucleotides, lipids, carbohydrates, and small molecules. The …

Mass spectrometry (MS) is a powerful tool to analyze complex mixtures of proteins in a high‐
throughput fashion. Proteome analysis has already become a routine task in biomedical …

Small, soluble metabolites not only are essential intermediates in intracellular biochemical
processes, but can also influence neighbouring cells when released into the extracellular …

INTRODUCTION There is a dimension to postnatal human development that involves
assembly of microbial communities in different body habitats, including the gut. Children with …

Mass spectrometry has transformed the field of cell signaling by enabling global studies of
dynamic protein phosphorylation ('phosphoproteomics'). Recent developments are enabling …

Robust, efficient, and reproducible protein extraction and sample processing is a key step for
bottom-up proteomics analyses. While many sample preparation protocols for mass …

Mass spectrometry (MS)-based proteomics typically employs multistep sample-preparation
workflows that are subject to sample contamination and loss. We report an in-StageTip …

A role for fibroblasts in physiological and pathological angiogenesis is now well recognized;
however, the precise mechanisms underlying their action have not been determined. Using …

The integrity of quantitative proteomic experiments depends on the reliability and the
robustness of the protein extraction, solubilization, and digestion methods utilized …

Alternative splicing events are a major causal mechanism for complex traits, but they have
been understudied due to the limitation of short-read sequencing. Here, we generate a full …