Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

By ecologically interacting with various biotic and abiotic agents acting in soil ecosystems,
highly diverse soil microorganisms establish complex and stable assemblages and survive …

Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …

The long-read amplicon provides a species-level solution for the community. With the
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …

Persistent colonization and outgrowth of potentially pathogenic organisms in the intestine
can result from long-term antibiotic use or inflammatory conditions, and may perpetuate …

Abstract 16S ribosomal RNA-based analysis is the established standard for elucidating the
composition of microbial communities. While short-read 16S rRNA analyses are largely …

Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

The oral cavity is the habitat of several hundreds of microbial taxa that have evolved to
coexist in multispecies communities in this unique ecosystem. By contrast, the internal tissue …

High-throughput amplicon sequencing of large genomic regions remains challenging for
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …