Third-generation sequencing: the spearhead towards the radical transformation of modern genomics
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
Microbial interactions within multiple-strain biological control agents impact soil-borne plant disease
Major losses of crop yield and quality caused by soil-borne plant diseases have long
threatened the ecology and economy of agriculture and forestry. Biological control using …
threatened the ecology and economy of agriculture and forestry. Biological control using …
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …
decades. However, high-throughput sequencing of the full gene has only recently become a …
Primer, pipelines, parameters: issues in 16S rRNA gene sequencing
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …
investigating microbiomes. However, comparative studies on differences in procedures are …
Commensal consortia decolonize Enterobacteriaceae via ecological control
Persistent colonization and outgrowth of potentially pathogenic organisms in the intestine
can result from long-term antibiotic use or inflammatory conditions, and may perpetuate …
can result from long-term antibiotic use or inflammatory conditions, and may perpetuate …
Perspectives and benefits of high-throughput long-read sequencing in microbial ecology
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …
insights into microbial ecology and function. Yet, in many instances short-read HTS …
Microbial community-driven etiopathogenesis of peri-implantitis
Osseointegrated dental implants are a revolutionary tool in the armament of reconstructive
dentistry, employed to replace missing teeth and restore masticatory, occlusal, and esthetic …
dentistry, employed to replace missing teeth and restore masticatory, occlusal, and esthetic …
High-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing
High-throughput amplicon sequencing of large genomic regions remains challenging for
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …
Interpretations of environmental microbial community studies are biased by the selected 16S rRNA (gene) amplicon sequencing pipeline
One of the major methods to identify microbial community composition, to unravel microbial
population dynamics, and to explore microbial diversity in environmental samples is high …
population dynamics, and to explore microbial diversity in environmental samples is high …
Emu: species-level microbial community profiling of full-length 16S rRNA Oxford Nanopore sequencing data
Abstract 16S ribosomal RNA-based analysis is the established standard for elucidating the
composition of microbial communities. While short-read 16S rRNA analyses are largely …
composition of microbial communities. While short-read 16S rRNA analyses are largely …