Structural biology of TRP channels

UA Hellmich, R Gaudet - … Potential (TRP) Cation Channels: Volume II, 2014 - Springer
Membrane proteins remain challenging targets for structural biologists, despite recent
technical developments regarding sample preparation and structure determination. We …

Strategies for successful over-expression of human membrane transport systems using bacterial hosts: Future perspectives

M Galluccio, L Console, L Pochini, M Scalise… - International Journal of …, 2022 - mdpi.com
Ten percent of human genes encode for membrane transport systems, which are key
components in maintaining cell homeostasis. They are involved in the transport of nutrients …

Structural biology workflow for the expression and characterization of functional human sodium glucose transporter type 1 in Pichia pastoris

A Suades, A Alcaraz, E Cruz, E Álvarez-Marimon… - Scientific reports, 2019 - nature.com
Heterologous expression of human membrane proteins is a challenge in structural biology
towards drug discovery. Here we report a complete expression and purification process of a …

Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

L Zhang, K Wang, DA Klaerke, K Calloe, L Lowrey… - Cells, 2019 - mdpi.com
(1) Background: Human transient receptor potential (TRP) channels constitute a large family
of ion-conducting membrane proteins that allow the sensation of environmental cues. As the …

Different ligands of the TRPV3 cation channel cause distinct conformational changes as revealed by intrinsic tryptophan fluorescence quenching

B Billen, M Brams, S Debaveye, A Remeeva… - Journal of Biological …, 2015 - ASBMB
TRPV3 is a thermosensitive ion channel primarily expressed in epithelial tissues of the skin,
nose, and tongue. The channel has been implicated in environmental thermosensation …

Temperature-Dependent Activation of Thermosensitive Transient Receptor Potential Channels

K Uchida - Thermal Biology: Temperature Sensing and …, 2024 - Springer
Temperature detection is essential for the survival and perpetuation of any species.
Thermoreceptors in the skin sense the body temperature and also the temperatures of the …

Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies

B Heim, R Handrick, MD Hartmann, H Kiefer - Plos one, 2021 - journals.plos.org
Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis,
we present a generic approach to assess and optimize refolding yield through …

Sodium channel inhibitors

AK Vellatt, J McCafferty, SB Surade… - US Patent …, 2024 - Google Patents
2020-01-14 Assigned to IONTAS LIMITED reassignment IONTAS LIMITED ASSIGNMENT
OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BELL, Damian …

TRPC6 Channel Complex: Identification of Novel Regulatory Subunits in Human Platelets

T Bentrcia - 2020 - publikationen.sulb.uni-saarland.de
The canonical transient receptor potential channel 6 (TRPC6) is a Ca2+ permeable cation
channel. To identify the TRPC6 protein containing complex in human platelets, I used …

Sodium channel inhibitors

AK VELLATT, J Mccafferty, SB SURADE, T LUETKENS… - 2019 - Google Patents
MJMLBAPXMAOKDU-UHFFFAOYSA-N Huwentoxin IV Chemical compound C1SSCC (C
(NC (CSSCC (NC (= O) C (N) CCC (O)= O) C (= O) NC (CC (C) C) C (= O) NC (CCC (O)= O) …