Although deconvolution can improve the quality of any type of microscope, the high
computational time required has so far limited its massive spreading. Here we demonstrate …

Monitoring and visualizing specimens at a large penetration depth is a challenge. At depths
of hundreds of microns, several physical effects (such as, scattering, PSF distortion and …

We present a technique for improving the spatial resolution of two-photon excitation
microscopy; our technique combines annular illumination with an in situ estimation of the …

Time-gated detection, namely, only collecting the fluorescence photons after a time-delay
from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated …

In vivo imaging of cells gives a glimpse into the world of biology in a natural setting
unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has …

Multifunctional light sheet imaging flow cytometry of a large population of live cells at high
throughput is challenging and requires new technological advancement. Existing cytometry …

Molecular assembly in a complex cellular environment is vital for understanding underlying
biological mechanisms. Biophysical parameters (such as single-molecule cluster density …

The methods of image deconvolution are important for improving the quality of the detected
images in the different modalities of fluorescence microscopy such as wide‐field, confocal …

Polarization plays a crucial role in understanding the interaction of fluorescent molecules in
a light field. We report the study on the effect of a field–dipole interaction under polarization …

This work considers the propagation of high-frequency waves in highly scattering media
where physical absorption of a nonlinear nature occurs. Using the classical tools of the …