The diverse functions of GAPDH: views from different subcellular compartments

C Tristan, N Shahani, TW Sedlak, A Sawa - Cellular signalling, 2011 - Elsevier
Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently
appreciated. In addition to the cytoplasm where the majority of GAPDH is located under the …

Control selection for RNA quantitation

T Suzuki, PJ Higgins, DR Crawford - Biotechniques, 2000 - Taylor & Francis
The study of mammalian gene expression is often carried out at the level of mRNA. In such
analyses, one usually measures the amount of an mRNA of interest under different …

Housekee** genes as internal standards: use and limits

O Thellin, W Zorzi, B Lakaye, B De Borman… - Journal of …, 1999 - Elsevier
Quantitative studies are commonly realised in the biomedical research to compare RNA
expression in different experimental or clinical conditions. These quantifications are …

Validation of housekee** genes as internal control for studying gene expression in rice by quantitative real-time PCR

M Jain, A Nijhawan, AK Tyagi, JP Khurana - Biochemical and biophysical …, 2006 - Elsevier
For accurate and reliable gene expression results, normalization of real-time PCR data is
required against a control gene, which displays highly uniform expression in living …

Selection of reliable reference genes for gene expression studies in peach using real-time PCR

Z Tong, Z Gao, F Wang, J Zhou, Z Zhang - BMC molecular biology, 2009 - Springer
Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase

MA Sirover - Biochimica et Biophysica Acta (BBA)-Protein Structure …, 1999 - Elsevier
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was considered a classical
glycolytic protein examined for its pivotal role in energy production. It was also used as a …

GAPDH: a common enzyme with uncommon functions

C Nicholls, H Li, JP Liu - Clinical and Experimental …, 2012 - Wiley Online Library
Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) has long been recognized as an
important enzyme for energy metabolism and the production of ATP and pyruvate through …

Validation of internal control for gene expression study in soybean by quantitative real-time PCR

B Jian, B Liu, Y Bi, W Hou, C Wu, T Han - BMC molecular biology, 2008 - Springer
Background Normalizing to housekee** gene (HKG) can make results from quantitative
real-time PCR (qRT-PCR) more reliable. Recent studies have shown that no single HKG is …

Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis

N Sen, MR Hara, MD Kornberg, MB Cascio, BI Bae… - Nature cell …, 2008 - nature.com
Besides its role in glycolysis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
initiates a cell death cascade,,,,,,,,. Diverse apoptotic stimuli activate inducible nitric oxide …

Novel roles for GAPDH in cell death and carcinogenesis

A Colell, DR Green, JE Ricci - Cell Death & Differentiation, 2009 - nature.com
Growing evidence points to the fact that glucose metabolism has a central role in
carcinogenesis. Among the enzymes controlling this energy production pathway …