Regulation of peptidoglycan synthesis and remodelling

AJF Egan, J Errington, W Vollmer - Nature Reviews Microbiology, 2020‏ - nature.com
Bacteria surround their cell membrane with a net-like peptidoglycan layer, called sacculus,
to protect the cell from bursting and maintain its cell shape. Sacculus growth during …

Growth and division of the peptidoglycan matrix

PDA Rohs, TG Bernhardt - Annual review of microbiology, 2021‏ - annualreviews.org
Most bacteria are surrounded by a peptidoglycan cell wall that defines their shape and
protects them from osmotic lysis. The expansion and division of this structure therefore plays …

Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)

RML Queiroz, T Smith, E Villanueva… - Nature …, 2019‏ - nature.com
Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on
capture of polyadenylated RNAs and cannot recover proteins that interact with …

Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division

AW Bisson-Filho, YP Hsu, GR Squyres, E Kuru, F Wu… - Science, 2017‏ - science.org
The mechanism by which bacteria divide is not well understood. Cell division is mediated by
filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to …

SEDS proteins are a widespread family of bacterial cell wall polymerases

AJ Meeske, EP Riley, WP Robins, T Uehara… - Nature, 2016‏ - nature.com
Elongation of rod-shaped bacteria is mediated by a dynamic peptidoglycan-synthetizing
machinery called the Rod complex. Here we report that, in Bacillus subtilis, this complex is …

GTPase activity–coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis

X Yang, Z Lyu, A Miguel, R McQuillen, KC Huang… - Science, 2017‏ - science.org
The bacterial tubulin FtsZ is the central component of the cell division machinery,
coordinating an ensemble of proteins involved in septal cell wall synthesis to ensure …

DeepBacs for multi-task bacterial image analysis using open-source deep learning approaches

C Spahn, E Gómez-de-Mariscal, RF Laine… - Communications …, 2022‏ - nature.com
This work demonstrates and guides how to use a range of state-of-the-art artificial neural-
networks to analyse bacterial microscopy images using the recently developed …

Cell wall synthesis and remodelling dynamics determine division site architecture and cell shape in Escherichia coli

PP Navarro, A Vettiger, VY Ananda, PM Llopis… - Nature …, 2022‏ - nature.com
The bacterial division apparatus catalyses the synthesis and remodelling of septal
peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles …

A guide to single-particle tracking

F Simon, LE Weiss, S van Teeffelen - Nature Reviews Methods Primers, 2024‏ - nature.com
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

From the regulation of peptidoglycan synthesis to bacterial growth and morphology

A Typas, M Banzhaf, CA Gross… - Nature Reviews …, 2012‏ - nature.com
How bacteria grow and divide while retaining a defined shape is a fundamental question in
microbiology, but technological advances are now driving a new understanding of how the …