Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology

CJ Smith, AM Osborn - FEMS microbiology ecology, 2009 - academic.oup.com
Abstract Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in
microbial ecology to quantify the abundance and expression of taxonomic and functional …

Emerging patterns of marine nitrogen fixation

JA Sohm, EA Webb, DG Capone - Nature Reviews Microbiology, 2011 - nature.com
Biological N2 fixation is an important part of the marine nitrogen cycle as it provides a source
of new nitrogen that can support biological carbon export and sequestration. Research in …

[HTML][HTML] Global oceanic diazotroph database version 2 and elevated estimate of global oceanic N fixation

Z Shao, Y Xu, H Wang, W Luo, L Wang… - Earth System …, 2023 - essd.copernicus.org
Marine diazotrophs convert dinitrogen (N 2) gas into bioavailable nitrogen (N), supporting
life in the global ocean. In 2012, the first version of the global oceanic diazotroph database …

Nitrogen-fixing populations of Planctomycetes and Proteobacteria are abundant in surface ocean metagenomes

TO Delmont, C Quince, A Shaiber, ÖC Esen… - Nature …, 2018 - nature.com
Nitrogen fixation in the surface ocean impacts global marine nitrogen bioavailability and
thus microbial primary productivity. Until now, cyanobacterial populations have been viewed …

Unicellular cyanobacterium symbiotic with a single-celled eukaryotic alga

AW Thompson, RA Foster, A Krupke, BJ Carter… - Science, 2012 - science.org
Symbioses between nitrogen (N) 2–fixing prokaryotes and photosynthetic eukaryotes are
important for nitrogen acquisition in N-limited environments. Recently, a widely distributed …

Database of diazotrophs in global ocean: abundance, biomass and nitrogen fixation rates

YW Luo, SC Doney, LA Anderson… - Earth System …, 2012 - essd.copernicus.org
Marine N 2 fixing microorganisms, termed diazotrophs, are a key functional group in marine
pelagic ecosystems. The biological fixation of dinitrogen (N 2) to bioavailable nitrogen …

The small unicellular diazotrophic symbiont, UCYN-A, is a key player in the marine nitrogen cycle

C Martínez-Pérez, W Mohr, CR Löscher… - Nature …, 2016 - nature.com
Abstract Microbial dinitrogen (N 2) fixation, the nitrogenase enzyme-catalysed reduction of N
2 gas into biologically available ammonia, is the main source of new nitrogen (N) in the …

Nitrogen cycle of the open ocean: from genes to ecosystems

JP Zehr, RM Kudela - Annual review of marine science, 2011 - annualreviews.org
The marine nitrogen (N) cycle controls the productivity of the oceans. This cycle is driven by
complex biogeochemical transformations, including nitrogen fixation, denitrification, and …

Methodological underestimation of oceanic nitrogen fixation rates

W Mohr, T Großkopf, DWR Wallace, J LaRoche - PloS one, 2010 - journals.plos.org
The two commonly applied methods to assess dinitrogen (N2) fixation rates are the 15N2-
tracer addition and the acetylene reduction assay (ARA). Discrepancies between the two …

Nutrient regulation of biological nitrogen fixation across the tropical western North Pacific

Z Wen, TJ Browning, Y Cai, R Dai, R Zhang, C Du… - Science …, 2022 - science.org
Nitrogen fixation is critical for the biological productivity of the ocean, but clear mechanistic
controls on this process remain elusive. Here, we investigate the abundance, activity, and …