The endothelium, a key actor in organ development and hPSC-derived organoid vascularization

A Vargas-Valderrama, A Messina… - Journal of Biomedical …, 2020 - Springer
Over the last 4 decades, cell culture techniques have evolved towards the creation of in vitro
multicellular entities that incorporate the three-dimensional complexity of in vivo tissues and …

Current perspective: 3D spheroid models utilizing human-based cells for investigating metabolism-dependent drug-induced liver injury

CR Cox, S Lynch, C Goldring… - Frontiers in medical …, 2020 - frontiersin.org
Drug-induced liver injury (DILI) remains a leading cause for the withdrawal of approved
drugs. This has significant financial implications for pharmaceutical companies, places …

Engineering neurovascular organoids with 3D printed microfluidic chips

I Salmon, S Grebenyuk, ARA Fattah, G Rustandi… - Lab on a Chip, 2022 - pubs.rsc.org
The generation of tissue and organs requires close interaction with vasculature from the
earliest moments of embryonic development. Tissue-specific organoids derived from …

[HTML][HTML] A human liver organoid screening platform for DILI risk prediction

CJ Zhang, SR Meyer, MJ O'Meara, S Huang… - Journal of …, 2023 - Elsevier
Background & Aims Drug-induced liver injury (DILI), both intrinsic and idiosyncratic, causes
frequent morbidity, mortality, clinical trial failures and post-approval withdrawal. This …

3D bioprinting of liver spheroids derived from human induced pluripotent stem cells sustain liver function and viability in vitro

E Goulart, LC de Caires-Junior, KA Telles-Silva… - …, 2019 - iopscience.iop.org
The liver is responsible for many metabolic, endocrine and exocrine functions.
Approximately 2 million deaths per year are associated with liver failure. Modern 3D …

Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling

HJ Kim, G Kim, KY Chi, H Kim, YJ Jang, S Jo… - Stem Cell Research & …, 2023 - Springer
Background The generation of liver organoids recapitulating parenchymal and non-
parenchymal cell interplay is essential for the precise in vitro modeling of liver diseases …

[HTML][HTML] Evolution of organoid technology: Lessons learnt in Co-Culture systems from developmental biology

E Zahmatkesh, N Khoshdel-Rad, H Mirzaei… - Developmental …, 2021 - Elsevier
In recent years, the development of 3D organoids has opened new avenues of investigation
into development, physiology, and regenerative medicine. Organoid formation and the …

Advancements in stem cell-derived hepatocyte-like cell models for hepatotoxicity testing

M **, X Yi, W Liao, Q Chen, W Yang, Y Li, S Li… - Stem cell research & …, 2021 - Springer
Drug-induced liver injury (DILI) is one of the leading causes of clinical trial failures and high
drug attrition rates. Currently, the commonly used hepatocyte models include primary human …

Do not keep it simple: recent advances in the generation of complex organoids

P Wörsdörfer, TI, I Asahina, Y Sumita… - Journal of Neural …, 2020 - Springer
Abstract 3D cell culture models which closely resemble real human tissues are of high
interest for disease modelling, drug screening as well as a deeper understanding of human …

Challenges for the applications of human pluripotent stem cell-derived liver organoids

M Chang, MS Bogacheva, YR Lou - Frontiers in Cell and …, 2021 - frontiersin.org
The current organoid culture systems allow pluripotent and adult stem cells to self-organize
to form three-dimensional (3D) structures that provide a faithful recapitulation of the …