Engineering strategies to optimize lignocellulosic biorefineries

B Long, F Zhang, SY Dai, M Foston, YJ Tang… - Nature Reviews …, 2024 - nature.com
Lignocellulosic biorefineries may be applied to produce value-added products, such as
chemicals, biofuels and bioplastics, from biomass, thereby reducing carbon emissions …

Kluyveromyces as promising yeast cell factories for industrial bioproduction: From bio-functional design to applications

Y Qiu, P Lei, R Wang, L Sun, Z Luo, S Li, H Xu - Biotechnology Advances, 2023 - Elsevier
As the two most widely used Kluyveromyces yeast, Kluyveromyces marxianus and K. lactis
have gained increasing attention as microbial chassis in biocatalysts, biomanufacturing and …

Densification pretreatment with a limited deep eutectic solvent triggers high-efficiency fractionation and valorization of lignocellulose

G Shen, X Yuan, Y Cheng, S Chen, Z Xu, M ** - Green Chemistry, 2023 - pubs.rsc.org
Deep eutectic solvents (DESs) are intriguing for biomass fractionation, but high DES dosage
and low biomass loading make them challenging to be used in industry. In this study, a DES …

High titer (> 200 g/L) lactic acid production from undetoxified pretreated corn stover

Y Zhang, Z Xu, M Lu, X Ma, S Chen, Y Wang… - Bioresource …, 2023 - Elsevier
Lignocellulosic biomass is a reliable feedstock for lactic acid fermentation, low product titers
hamper the scale production of cellulosic lactic acid. In this study, a Densifying …

Efficient co-production of xylo-oligosaccharides and probiotics from corncob by combined lactic acid pretreatment and two-step enzymatic hydrolysis

Q Yang, L Zhang, Z Lian, J Zhang - Bioresource Technology, 2023 - Elsevier
Lactic acid (LA) is efficient in xylo-oligosaccharides (XOS) production from poplar. However,
the role of LA in XOS production from corncob has not been carefully elucidated, and the co …

Efficient 2-O-α-D-glucopyranosyl-sn-glycerol production by single whole-cell biotransformation through combined engineering and expression regulation with novel …

P Duan, M Long, X Zhang, Z Liu, J You, X Pan… - Bioresource …, 2023 - Elsevier
O-α-D-glucopyranosyl-sn-glycerol (2-αGG) is a high value product with wide applications.
Here, an efficient, safe and sustainable bioprocesses for 2-αGG production was designed. A …

[HTML][HTML] Impact of ancestral sequence reconstruction on mechanistic and structural enzymology

CR Nicoll, M Massari, MW Fraaije, ML Mascotti… - Current Opinion in …, 2023 - Elsevier
Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein
sequence across evolution. More specifically, it can illustrate how specific amino acid …

Structure, rheology, and antifreeze property of the exopolysaccharide from Naematelia aurantialba through basidiospore fermentation

T Sun, X Xu, Y Ma, K Yang, R Wang, Y Gu, S Li, Y Qiu… - Food …, 2023 - Elsevier
The quality of frozen food can be maintained with antifreeze. Commercial antifreezes like
sucrose and glycerol are ineffective and may pose health hazards, making them …

[HTML][HTML] Engineering Saccharomyces cerevisiae for application in integrated bioprocessing biorefineries

LS Minnaar, F Kruger, J Fortuin, LJ Hoffmeester… - Current Opinion in …, 2024 - Elsevier
Highlights•Consolidated bioprocessing conversion of cellulose to ethanol is not yet
viable.•The low value of energy products has shifted the focus to higher-value commodities.• …

Engineering the xylose metabolism of Saccharomyces cerevisiae for ethanol and single cell protein bioconversion

M Huang, Z **, H Ni, P Zhang, H Li, J Liu, C Weng… - Biomass and …, 2024 - Elsevier
Xylose isomerase (XI) pathway has been widely employed to enable Saccharomyces
cerevisiae to convert xylose and glucose into commercially feasible lignocellulosic ethanol …